caa a22 4500 475767063 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s007050070079 doi (NATIONALLICENCE)springer-10.1007/s007050070079 Categorization of North American porcine reproductive and respiratory syndrome viruses: Epitopic profiles of the N, M, GP5 and GP3 proteins and susceptibility to neutralization [Elektronische Daten] [L. Yang, M. L. Frey, K.-J. Yoon, J. J. Zimmerman, K. B. Platt] Summary.:   Eleven epitopes were identified by murine monoclonal antibodies (MAbs) that represented the N, M, GP5 and GP3 proteins of the North American (NA) porcine reproductive and respiratory syndrome (PRRS) virus, KY 35 (NVSL 46907). Three discontinuous epitopes of the N and M proteins were designated EpORF7-Fd through Hd and EpORF6-Ad through Cd. Five continuous epitopes of the GP5 and GP3 proteins were designated EpORF5-A through C and EpORF3-A and B. The MAbs representing EpORF5-C and EpORF6-A and B had neutralizing activity. The MAbs representing the above epitopes, except EpORF7-Gd and Hd, expanded the virus marker system described in a previous study in which a panel of 69 NA viruses and the Lelystad virus were categorized into 5 antigenic groups, I15 through V15 based on the presence or absence of 5 continuous epitopes of the N protein. Antigenic groups I15 and II15, which represented 84.7 and 11.6% of all viruses tested, were categorized further into 9 and 4 subgroups, respectively. The remaining NA viruses and the Lelystad virus were distributed among 4 groups, one of which was represented by 2 subgroups. Significant (P<0.05) differences in sensitivity to neutralization of 28 viruses representing 6 antigenic groups by the 3 neutralizing MAbs suggested that sensitivity to neutralization may also be of value in categorizing PRRS viruses. Springer-Verlag/Wien, 2000 Yang L. Department of Veterinary Microbiology and Preventive Medicine College of Veterinary Medicine, Iowa State University, Ames, Iowa, U.S.A., US aut Frey M. L. Department of Veterinary Diagnostic and Production Animal Medicine College of Veterinary Medicine, Iowa State University, Ames, Iowa, U.S.A., US aut Yoon K.-J Department of Veterinary Diagnostic and Production Animal Medicine College of Veterinary Medicine, Iowa State University, Ames, Iowa, U.S.A., US aut Zimmerman J. J. Department of Veterinary Diagnostic and Production Animal Medicine College of Veterinary Medicine, Iowa State University, Ames, Iowa, U.S.A., US aut Platt K. B. Department of Veterinary Microbiology and Preventive Medicine College of Veterinary Medicine, Iowa State University, Ames, Iowa, U.S.A., US aut https://doi.org/10.1007/s007050070079 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070079 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang L. Department of Veterinary Microbiology and Preventive Medicine College of Veterinary Medicine, Iowa State University, Ames, Iowa, U.S.A., US aut NATIONALLICENCE 700 1- Frey M. L. Department of Veterinary Diagnostic and Production Animal Medicine College of Veterinary Medicine, Iowa State University, Ames, Iowa, U.S.A., US aut NATIONALLICENCE 700 1- Yoon K.-J Department of Veterinary Diagnostic and Production Animal Medicine College of Veterinary Medicine, Iowa State University, Ames, Iowa, U.S.A., US aut NATIONALLICENCE 700 1- Zimmerman J. J. Department of Veterinary Diagnostic and Production Animal Medicine College of Veterinary Medicine, Iowa State University, Ames, Iowa, U.S.A., US aut NATIONALLICENCE 700 1- Platt K. B. Department of Veterinary Microbiology and Preventive Medicine College of Veterinary Medicine, Iowa State University, Ames, Iowa, U.S.A., US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer