caa a22 4500 475767071 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s007050070077 doi (NATIONALLICENCE)springer-10.1007/s007050070077 In vitro antiviral activity of lactoferrin and ribavirin upon hantavirus [Elektronische Daten] [M. E. Murphy, H. Kariwa, T. Mizutani, K. Yoshimatsu, J. Arikawa, I. Takashima] Summary.:  Bovine lactoferrin (LF) and ribavirin (Rbv) were tested as antiviral agents against Seoul type hantavirus (SR-11 strain) in vitro. Hantaviral foci number in Vero E6 cells infected with SR-11 was reduced with LF treatment by 5 days post infection to obtain a 50% effective dose (ED50) of 2500 μg/ml, while pretreatment with LF was highly efficacious having an ED50 of 39 μg/ml. Conversely, 1 h pretreatment with Rbv revealed no inhibition of viral focus formation but could significantly reduce the number of viral foci (ED50: 10 μg/ml) when used from the time of viral infection. One hour pre-treatment of the cell monolayer with LF and subsequent addition of Rbv revealed a synergistic anti-hantaviral effect against SR-11, <20 FFU/ml as compared to 105 foci/ml in the control. One hour treatment of SR-11 with LF prior to cell inoculation gave an ED50 of 312.5 μg/ml. Whereas, washing the LF-pretreated cell monolayer with PBS demonstrated minimal focus reduction, suggesting LF lightly adheres to cells. These results indicate that LF has anti-hantaviral activity in vitro and inhibition of virus adsorption to cells which play an important role in revealing the anti-hantaviral activity of LF. This paper reports for the first time the anti-hantaviral effect of LF. Springer-Verlag/Wien, 2000 Murphy M. E. Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut Kariwa H. Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut Mizutani T. Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut Yoshimatsu K. Institute for Animal Experimentation, School of Medicine, Hokkaido University, Sapporo, Japan, JP aut Arikawa J. Institute for Animal Experimentation, School of Medicine, Hokkaido University, Sapporo, Japan, JP aut Takashima I. Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut https://doi.org/10.1007/s007050070077 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070077 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Murphy M. E. Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 700 1- Kariwa H. Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 700 1- Mizutani T. Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 700 1- Yoshimatsu K. Institute for Animal Experimentation, School of Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 700 1- Arikawa J. Institute for Animal Experimentation, School of Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 700 1- Takashima I. Laboratory of Public Health, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer