caa a22 4500 475767098 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s007050050682 doi (NATIONALLICENCE)springer-10.1007/s007050050682 Epitope mapping of the influenza A virus RNA polymerase PA using monoclonal antibodies [Elektronische Daten] [M. Hatta, Y. Asano, K. Masunaga, T. Ito, K. Okazaki, T. Toyoda, Y. Kawaoka, A. Ishihama, H. Kida] Summary.:  To obtain reagents to functionally map the PA protein, we produced monoclonal antibodies specific to this protein. Twenty-two monoclonal antibodies reacting with PA protein in ELISA were divided into 10 groups on the basis of competitive binding patterns to this protein. Of these, seventeen monoclonal antibodies bound to PA polypeptide spanning amino acids 101-400 and three bound to that of amino acids 518-600, while the other two did not react with any PA polypeptides tested with the exception of full-length PA. Among these monoclonal antibodies, only five reacted with PA in A/PR/8/34 virus-infected cells in indirect immunofluorescence assay. Thus, we obtained monoclonal antibodies that recognize at least 10 distinct regions of the PA molecule. These monoclonal antibodies should be useful in dissecting functions of the PA protein. Springer-Verlag, 2000 Hatta M. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut Asano Y. Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan, JP aut Masunaga K. Department of Virology, Kurume University School of Medicine, Kurume, Fukuoka, Japan, JP aut Ito T. Department of Veterinary Public Health, Faculty of Agriculture, Tottori University, Tottori, Japan, JP aut Okazaki K. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut Toyoda T. Department of Virology, Kurume University School of Medicine, Kurume, Fukuoka, Japan, JP aut Kawaoka Y. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, U.S.A., US aut Ishihama A. Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan, JP aut Kida H. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut Archives of Virology Springer-Verlag 145/5(2000-05-01), 895-903 0304-8608 145:5<895 2000 145 705 https://doi.org/10.1007/s007050050682 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050682 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hatta M. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 700 1- Asano Y. Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan, JP aut NATIONALLICENCE 700 1- Masunaga K. Department of Virology, Kurume University School of Medicine, Kurume, Fukuoka, Japan, JP aut NATIONALLICENCE 700 1- Ito T. Department of Veterinary Public Health, Faculty of Agriculture, Tottori University, Tottori, Japan, JP aut NATIONALLICENCE 700 1- Okazaki K. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 700 1- Toyoda T. Department of Virology, Kurume University School of Medicine, Kurume, Fukuoka, Japan, JP aut NATIONALLICENCE 700 1- Kawaoka Y. Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, U.S.A., US aut NATIONALLICENCE 700 1- Ishihama A. Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan, JP aut NATIONALLICENCE 700 1- Kida H. Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/5(2000-05-01), 895-903 0304-8608 145:5<895 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence SWISSBIB 475767098 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer