caa a22 4500 475767101 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s007050050686 doi (NATIONALLICENCE)springer-10.1007/s007050050686 Identification and in vivo expression of a prokaryotic-like ribosome recognition sequence upstream of the coat protein gene of potato virus X [Elektronische Daten] [K. L. Hefferon, J. Xu, M. G. AbouHaidar] Summary.:  Conserved prokaryotic sequence motifs, distinct from the classic Shine-Dalgarno sequence, yet possessing homology to 16S rRNA in E. coli have been identified in a number of plant viruses. In this report, a similar Shine-Dalgarno-like motif located immediately upstream to the CP gene of potato virus X (PVX) was demonstrated to enable expression of a reporter gene in E. coli to approximately one third the level of a similar construct containing the classical Shine-Dalgarno sequence. Both PVX-specific CP and RNA transcripts were detected in chloroplasts purified from transgenic potato plants containing the PVX CP gene and corresponding leader sequence. Protoplasts generated from these transgenic plants were used to demonstrate that expression of the PVX CP from chloroplasts is possible. The implications of these results on the PVX infection cycle are discussed. Springer-Verlag, 2000 Hefferon K. L. Boyce Thompson Institute for Plant Research at Cornell University, Ithaca, New York, U.S.A., US aut Xu J. Departments of Botany and Microbiology, University of Toronto, Toronto, Ontario, Canada, CA aut AbouHaidar M. G. Departments of Botany and Microbiology, University of Toronto, Toronto, Ontario, Canada, CA aut Archives of Virology Springer-Verlag 145/5(2000-05-01), 945-956 0304-8608 145:5<945 2000 145 705 https://doi.org/10.1007/s007050050686 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050686 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hefferon K. L. Boyce Thompson Institute for Plant Research at Cornell University, Ithaca, New York, U.S.A., US aut NATIONALLICENCE 700 1- Xu J. Departments of Botany and Microbiology, University of Toronto, Toronto, Ontario, Canada, CA aut NATIONALLICENCE 700 1- AbouHaidar M. G. Departments of Botany and Microbiology, University of Toronto, Toronto, Ontario, Canada, CA aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/5(2000-05-01), 945-956 0304-8608 145:5<945 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer