caa a22 4500 47576711X CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s007050050690 doi (NATIONALLICENCE)springer-10.1007/s007050050690 Nucleotide sequence and genome organisation of cherry mottle leaf virus and its relationship to members of the Trichovirus genus [Elektronische Daten] [D. James, W. Jelkmann, C. Upton] Summary.:  The nucleotide sequence of cherry mottle leaf virus (CMLV) was determined and compared to sequences of a number of plant viruses including the type member of the Trichovirus genus (apple chlorotic leafspot virus, ACLSV), and members of the Vitivirus genus including grapevine virus B, (GVB). The CMLV genome was determined to consist of 8003 nt excluding the poly(A) tail at the 3′ end of the genome. The overall A+U content of CMLV genomic RNA was 59. 1%, which is similar to ACLSV, but significantly different from GVB. Four putative open reading frames were identified (ORFs 1, 2, 3, and 4) encoding proteins of Mr 215. 8 kDa, 47 kDa, 21.6 kDa, and 15. 3 kDa, respectively. This differs from ACLSV which has 3 ORFS, and GVB which has 5 ORFs. Protein database searches showed no matches of CMLV ORF4 with ACLSV sequences, but found similarities between ORF4 of CMLV and ORF5 of GVB, suggesting that this may be a nucleic acid-binding protein. CMLV and ACLSV formed a common virus clade in phylogenetic analysis of the coat protein amino acid sequence and except for CMLV's ORF4, these viruses show high levels of similarity throughout the genome. CMLV appears to be a member of the Trichovirus genus. Springer-Verlag, 2000 James D. Centre for Plant Health, Canadian Food Inspection Agency, Sidney, Britsh Columbia, Canada, CA aut Jelkmann W. Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenschutz im Obstbau, Dossenheim, Germany, DE aut Upton C. Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada, CA aut Archives of Virology Springer-Verlag 145/5(2000-05-01), 995-1007 0304-8608 145:5<995 2000 145 705 https://doi.org/10.1007/s007050050690 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050690 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- James D. Centre for Plant Health, Canadian Food Inspection Agency, Sidney, Britsh Columbia, Canada, CA aut NATIONALLICENCE 700 1- Jelkmann W. Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Pflanzenschutz im Obstbau, Dossenheim, Germany, DE aut NATIONALLICENCE 700 1- Upton C. Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada, CA aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/5(2000-05-01), 995-1007 0304-8608 145:5<995 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer