caa a22 4500 475767136 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s007050050689 doi (NATIONALLICENCE)springer-10.1007/s007050050689 Identification of a new human DNA virus (TTV-like mini virus, TLMV) intermediately related to TT virus and chicken anemia virus [Elektronische Daten] [K. Takahashi, Y. Iwasa, M. Hijikata, S. Mishiro] Summary.:  TT virus (TTV) is the only known human virus with single-stranded circular DNA, with a possible but yet unclear relationship to chicken anemia virus (CAV) of the family Circoviridae. Here we report a new human virus resembling TTV and CAV, designated TTV-like mini virus (TLMV). This non-enveloped virus was smaller (>30 nm) but had a similar density (1. 31-1.34 g/ml in CsCl) to TTV, when a TLMV/TTV-coinfected plasma was analyzed. Full-length sequencing revealed that the TLMV genome was a circular DNA comprising 2860 nt (isolate CBD231); significantly shorter than TTV (TA278, 3852 nt) but longer than CAV (CAECUX1, 2319 nt). A strand-specific hybridization assay using oligonucleotide-coated beads suggested TLMV was negative-stranded, like TTV and CAV. In genomic organization, TLMV was similar to both TTV and CAV. The untranslated region of TLMV resembled CAV in that both had direct repeats, whereas the sequence homology was more evident between TLMV and TTV. The predicted ORF1 protein of TLMV was rich in R/W/F residues at its amino terminus; the richness in W was shared by TTV, F by CAV, and R by both. ORF2 proteins of the three viruses had a common motif, WX7HX3CXCX5H. Thus, TLMV is an intermediate between the remotely related TTV and CAV. Since CAV differs much from other circoviruses, it may better be classified together with TTV and TLMV under a new family: we would coin the Paracircoviridae. Springer-Verlag, 2000 Takahashi K. Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan, JP aut Iwasa Y. Hepatitis Research Institute, Tokyo, Japan, JP aut Hijikata M. Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan, JP aut Mishiro S. Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan, JP aut Archives of Virology Springer-Verlag 145/5(2000-05-01), 979-993 0304-8608 145:5<979 2000 145 705 https://doi.org/10.1007/s007050050689 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050689 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi K. Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Iwasa Y. Hepatitis Research Institute, Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Hijikata M. Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Mishiro S. Department of Medical Sciences, Toshiba General Hospital, Tokyo, Japan, JP aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/5(2000-05-01), 979-993 0304-8608 145:5<979 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer