caa a22 4500 475767160 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s007050050684 doi (NATIONALLICENCE)springer-10.1007/s007050050684 Epitopes on glycoprotein E and on the glycoprotein E/glycoprotein I complex of bovine herpesvirus 1 are expressed by all of 222 isolates and 11 vaccine strains [Elektronische Daten] [F. A. M. Rijsewijk, M. J. Kaashoek, J. P. M. Langeveld, M. A. Maris-Veldhuis, J. Magdalena, S. B. E. Verschuren, R. H. Meloen, J. T. van Oirschot] Summary.:  Glycoprotein E (gE) of bovine herpesvirus 1 (BHV1) forms a complex with glycoprotein I (gI) and plays an important role in cell-to-cell spread mechanisms of the virus, but is not essential for propagation of the virus. To study the antigenic variability of BHV1 glycoprotein E, a set of six well characterised monoclonal antibodies (MAbs) was established using BHV1 gE and gI deletion mutants, eukaryotically expressed gE and gI and pepscan analysis. Two of these MAbs reacted with a linear gE epitope (MAbs 3 and 52), two reacted with a more conformation dependent gE epitope (MAbs 61 and 81) and two reacted with epitopes formed by a complex formed between gE and glycoprotein I (MAbs 67 and 75). With these six MAbs the gE expression of 222 BHV1 isolates and 11 BHV1 modified-live vaccine strains was studied in vitro, using an immunoperoxidase monolayer assay. All 222 BHV1 isolates and 11 vaccine strains were found to react with MAbs 61, 81 and 75. Three of the 222 isolates failed to react with MAb 67 and two of the vaccines reacted very weakly with MAbs 3 and 52. Analysis of the gE genes of these five aberrant isolates and the gE glycoproteins they expressed, did not show obvious size differences compared to wild-type BHV1. We conclude that the tested gE epitopes are highly conserved, including the epitopes formed by the gI/gE complex. Springer-Verlag, 2000 Rijsewijk F. A. M. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut Kaashoek M. J. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut Langeveld J. P. M. Institute for Animal Science and Health (ID-Lelystad), Department of Molecular Recognition, Lelystad, The Netherlands, NL aut Maris-Veldhuis M. A. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut Magdalena J. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut Verschuren S. B. E. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut Meloen R. H. Institute for Animal Science and Health (ID-Lelystad), Department of Molecular Recognition, Lelystad, The Netherlands, NL aut van Oirschot J. T. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut Archives of Virology Springer-Verlag 145/5(2000-05-01), 921-936 0304-8608 145:5<921 2000 145 705 https://doi.org/10.1007/s007050050684 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050684 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rijsewijk F. A. M. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut NATIONALLICENCE 700 1- Kaashoek M. J. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut NATIONALLICENCE 700 1- Langeveld J. P. M. Institute for Animal Science and Health (ID-Lelystad), Department of Molecular Recognition, Lelystad, The Netherlands, NL aut NATIONALLICENCE 700 1- Maris-Veldhuis M. A. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut NATIONALLICENCE 700 1- Magdalena J. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut NATIONALLICENCE 700 1- Verschuren S. B. E. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut NATIONALLICENCE 700 1- Meloen R. H. Institute for Animal Science and Health (ID-Lelystad), Department of Molecular Recognition, Lelystad, The Netherlands, NL aut NATIONALLICENCE 700 1- van Oirschot J. T. Institute for Animal Science and Health (ID-Lelystad), Department of Mammalian Virology, Lelystad, The Netherlands, NL aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/5(2000-05-01), 921-936 0304-8608 145:5<921 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer