caa a22 4500 475767187 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s007050050679 doi (NATIONALLICENCE)springer-10.1007/s007050050679 Investigation into the mode of action of R-91650, an arylpiperazinyl fluoroquinolone, on feline immunodeficiency virus replication inhibitory activity [Elektronische Daten] [H. Kashiwase, T. Katsube, K. Iida, T. Komai, T. Nishigaki, T. Kimura, M. Yamashita] Summary.:  The mode of action of R-91650, an arylpiperazinyl fluoroquinolone, on feline immunodeficiency virus (FIV) replication inhibitory activity was investigated. R-91650 inhibited replication of FIV at non-cytotoxic concentration levels in both acutely infected peripheral blood mononuclear cells and chronically infected P-CrFK cells. The compound reduced the intracellular p24 concentration levels in P-CrFK cells in a dose-dependent manner. Northern blot analysis revealed that R-91650 selectively prevented the accumulation of FIV mRNA in P-CrFK cells. However, the compound did not inhibit FIV-long terminal repeat (LTR) promoter activity in the reporter gene expression analysis. These data suggest that R-91650 is a novel inhibitor of FIV replication that inhibits a certain step or steps following transcription initiation of the FIV-LTR promoter. Springer-Verlag, 2000 Kashiwase H. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut Katsube T. Pharmaceutical Research Department, Ube Laboratories, Ube Industries Ltd., Ube City, Yamaguchi, Japan, JP aut Iida K. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut Komai T. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut Nishigaki T. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut Kimura T. Medicinal Chemistry Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut Yamashita M. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut Archives of Virology Springer-Verlag 145/5(2000-05-01), 859-869 0304-8608 145:5<859 2000 145 705 https://doi.org/10.1007/s007050050679 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050679 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kashiwase H. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Katsube T. Pharmaceutical Research Department, Ube Laboratories, Ube Industries Ltd., Ube City, Yamaguchi, Japan, JP aut NATIONALLICENCE 700 1- Iida K. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Komai T. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Nishigaki T. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Kimura T. Medicinal Chemistry Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Yamashita M. Biological Research Laboratories, Sankyo Co., Ltd., Tokyo, Japan, JP aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/5(2000-05-01), 859-869 0304-8608 145:5<859 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer