caa a22 4500 475767195 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s007050050691 doi (NATIONALLICENCE)springer-10.1007/s007050050691 Molecular evidence that sugarcane yellow leaf virus (ScYLV) is amember of the Luteoviridae family [Elektronische Daten] [I. G. Maia, M. C. Gonçalves, P. Arruda, J. Vega] Summary.:  A previously uncharacterized virus was reported in southeast Brazil causing a yellowing leaf disease in sugarcane. The virus, termed sugarcane yellow leaf virus (ScYLV), shares features typical of the luteoviruses. To start the molecular characterization of ScYLV, the nucleotide sequence of the coat protein (CP), 17 kDa protein and C-terminus of the RNA-dependent RNA polymerase coding regions was determined from an RT-PCR amplification product. Comparisons showed that the deduced amino acid sequences share a considerable degree of identity and similarity with corresponding sequences of known luteoviruses, thus clearly establishing ScYLV as a member of the family Luteoviridae. The authenticity of the CP open reading frame was confirmed by its expression in Escherichia coli. The recombinant CP positively reacted in immunoblot assays with polyclonal antibodies raised against native ScYLV. Furthermore, phylogenetic analyses also suggest that the 5′ and 3′ coding blocks of the ScYLV genome possess different taxonomic affinities within the Luteoviridae family, as does also the genome of soybean dwarf virus. Springer-Verlag, 2000 Maia I. G. Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, SP, Brazil, BR aut Gonçalves M. C. Departamento de Fisiologia Vegetal, Universidade Estadual de Campinas, Campinas, SP, Brazil, BR aut Arruda P. Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, SP, Brazil, BR aut Vega J. Departamento de Fisiologia Vegetal, Universidade Estadual de Campinas, Campinas, SP, Brazil, BR aut Archives of Virology Springer-Verlag 145/5(2000-05-01), 1009-1019 0304-8608 145:5<1009 2000 145 705 https://doi.org/10.1007/s007050050691 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050691 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maia I. G. Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, SP, Brazil, BR aut NATIONALLICENCE 700 1- Gonçalves M. C. Departamento de Fisiologia Vegetal, Universidade Estadual de Campinas, Campinas, SP, Brazil, BR aut NATIONALLICENCE 700 1- Arruda P. Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, SP, Brazil, BR aut NATIONALLICENCE 700 1- Vega J. Departamento de Fisiologia Vegetal, Universidade Estadual de Campinas, Campinas, SP, Brazil, BR aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/5(2000-05-01), 1009-1019 0304-8608 145:5<1009 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer