caa a22 4500 475767314 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s007050050032 doi (NATIONALLICENCE)springer-10.1007/s007050050032 Evidence for translation of VP3 of avian polyomavirus BFDV by leaky ribosomal scanning [Elektronische Daten] [Q. Liu, G. Hobom] Summary.:  Due to several incomplete splicing reactions, budgerigar fledgling disease virus (BFDV) late mature mRNAs are either bicistronic or polycistronic with an agnogene located upstream of viral protein (VP) genes. While the bicistronic mRNAs code for the vast majority of VP1, the polycistronic mRNAs contain the coding sequences of VP2, VP3, and VP1 (as the most distal cistron relative to VP2 and VP3). In this work, the translation initiation mechanism of VP3 was investigated in chicken embryo fibroblast cells by transfection of a series of BFDV mutant clones and transient reporter gene chloramphenicol acetyltransferase (CAT) expression assay, leading to the conclusion that BFDV VP3 was translated by leaky ribosomal scanning. Furthermore, thanks to the high sensitivity of CAT assay experiment, we were able to demonstrate that ribosomes could reach VP1-AUG and initiate translation after scanning through 900 nucleotides on the unspliced polycistronic mRNA. 2000 Springer-Verlag/, Wien Liu Q. Institute of Microbiology and Molecular Biology, University of Giessen, Giessen, Germany, DE aut Hobom G. Institute of Microbiology and Molecular Biology, University of Giessen, Giessen, Germany, DE aut https://doi.org/10.1007/s007050050032 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050032 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Q. Institute of Microbiology and Molecular Biology, University of Giessen, Giessen, Germany, DE aut NATIONALLICENCE 700 1- Hobom G. Institute of Microbiology and Molecular Biology, University of Giessen, Giessen, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer