caa a22 4500 475767330 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s007050050031 doi (NATIONALLICENCE)springer-10.1007/s007050050031 Infectious cDNA clones of two grapevine viruses [Elektronische Daten] [P. Saldarelli, M. Dell'Orco, A. Minafra] Summary.:  Full-length cDNA copies of the genomes of Grapevine virus A (GVA) and Grapevine virus B (GVB) under the control of bacteriophage T7 promoter have been synthesized, which were refractory to cloning in Escherichia coli. However, both transcribed cDNAs were infectious when mechanically inoculated to Nicotiana plants. A full-length cDNA copy of GVB was engineered in pCass2, a plasmid containing a partially duplicated copy of the Ca35S promoter, but was rather unstable in Escherichia coli. No infection of Nicotiana plants was obtained following mechanical inoculation but detached Nicotiana leaves, inoculated by particle bombardment, supported the multiplication of a GVB isolate seemingly identical to the wild-type used for cloning. Nicotiana seedling inoculated with sap expressed from these leaves became infected showing typical GVB symptoms. Transient transcription of Ca35S driven cDNA clones was also detected by RT-PCR in leaves of the grapevine hybrid LN33 following inoculation by particle bombardment. The availability of infectious cDNA clones of GVA and GVB constitutes a tool for the study of genome expression and pathogenesis, and for the ultimate establishment of the aetiological role of these viruses. 2000 Springer-Verlag/, Wien Saldarelli P. Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee and Dipartimento di Protezione delle Piante, Università degli Studi, Bari Italy, IT aut Dell'Orco M. Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee and Dipartimento di Protezione delle Piante, Università degli Studi, Bari Italy, IT aut Minafra A. Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee and Dipartimento di Protezione delle Piante, Università degli Studi, Bari Italy, IT aut https://doi.org/10.1007/s007050050031 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050031 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saldarelli P. Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee and Dipartimento di Protezione delle Piante, Università degli Studi, Bari Italy, IT aut NATIONALLICENCE 700 1- Dell'Orco M. Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee and Dipartimento di Protezione delle Piante, Università degli Studi, Bari Italy, IT aut NATIONALLICENCE 700 1- Minafra A. Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee and Dipartimento di Protezione delle Piante, Università degli Studi, Bari Italy, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer