caa a22 4500 475767349 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s007050050019 doi (NATIONALLICENCE)springer-10.1007/s007050050019 Molecular analysis of the prototype coxsackievirus B5 genome [Elektronische Daten] [A. M. Lindberg, C. Polacek] Summary.:  To facilitate studies of the phylogenetic relationship between enteroviruses, in particular the prototype strain (Faulkner) of coxsackievirus B5 (CVB5F) and other CVB5 isolates and to facilitate studies of the interactions between CVB5F and the target cell, the complete nucleotide sequence of the prototype has been determined. The complete sequence was collected from three overlapping reverse transcription polymerase chain reaction (RT-PCR) generated amplicons. Molecular analysis of the CVB5F genome verified that this strain is more related to other CVB5 isolates and to swine vesicular disease virus strains than to other enteroviruses. In addition, comparison of the amino acid sequence derived from the structural genes indicated a division of group B coxsackievirus into subgroups, where CVB1, CVB3 and CVB5 constitute one group, CVB2 and CVB4 from a second group and CVB6 prototype forms a branch of its own. This observation, supported by reports describing the interactions between CVB and the cell surface, may reflect that these subgroups of group B coxsackieviruses have evolved to use slightly different approaches to carry out the complete infectious cycle within the cell. 2000 Springer-Verlag/, Wien Lindberg A. M. Institute of Natural Sciences, University of Kalmar, Kalmar, Sweden, SE aut Polacek C. Institute of Natural Sciences, University of Kalmar, Kalmar, Sweden, SE aut https://doi.org/10.1007/s007050050019 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050019 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lindberg A. M. Institute of Natural Sciences, University of Kalmar, Kalmar, Sweden, SE aut NATIONALLICENCE 700 1- Polacek C. Institute of Natural Sciences, University of Kalmar, Kalmar, Sweden, SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer