caa a22 4500 475767357 CHVBK 20180406123609.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s007050050025 doi (NATIONALLICENCE)springer-10.1007/s007050050025 Comparative studies of piscine and amphibian iridoviruses [Elektronische Daten] [A. D. Hyatt, A. R. Gould, Z. Zupanovic, A. A. Cunningham, S. Hengstberger, R. J. Whittington, J. Kattenbelt, B. E. H. Coupar] Summary.:  A total of 30 iridoviruses collected from Australia, South-East Asia, North America, South America and Europe were characterised. With the exception of the South-East Asian iridoviruses all viruses were found to belong to the genus Ranavirus. All viruses, except those originating from South-East Asia, cross-reacted with antisera against epizootic haematopoietic necrosis virus (EHNV). Viruses or virus-infected cells were examined using electron microscopy, SDS PAGE, restriction endonuclease (RE) digestion, DNA hybridisation, and DNA sequencing. Data from RE digestion of genomic DNA, and from the sequencing of PCR products indicated that the viruses generally grouped according to their geographic and taxonomic (i.e. amphibian or fish) origin. The one exception to this was the viruses from the United Kingdom that grouped with the North American ranaviruses. The differences between specified genomic regions were small. To assess the validity of the differences in sequence homology, similar studies were performed with different isolates from two viruses (EHNV and Guatopo virus (GV), collected from different animals at different locations and time). The sequence data showed complete homology for the isolates for any one virus over the 200 and 586 bp regions examined. Collectively, the data showed that the coding region for the major coat protein (MCP) is stable for any one species (e.g. EHNV). 2000 Springer-Verlag/, Wien Hyatt A. D. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut Gould A. R. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut Zupanovic Z. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut Cunningham A. A. Institute of Zoology, London, U.K., GB aut Hengstberger S. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut Whittington R. J. New South Wales, Elizabeth Macarthur Agricultural Institute, Camden, Australia, AU aut Kattenbelt J. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut Coupar B. E. H. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut https://doi.org/10.1007/s007050050025 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050025 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hyatt A. D. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut NATIONALLICENCE 700 1- Gould A. R. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut NATIONALLICENCE 700 1- Zupanovic Z. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut NATIONALLICENCE 700 1- Cunningham A. A. Institute of Zoology, London, U.K., GB aut NATIONALLICENCE 700 1- Hengstberger S. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut NATIONALLICENCE 700 1- Whittington R. J. New South Wales, Elizabeth Macarthur Agricultural Institute, Camden, Australia, AU aut NATIONALLICENCE 700 1- Kattenbelt J. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut NATIONALLICENCE 700 1- Coupar B. E. H. Australian Animal Health Laboratory, CSIRO, Geelong, Victoria, Australia, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer