caa a22 4500 47576739X CHVBK 20180406123610.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s007050050030 doi (NATIONALLICENCE)springer-10.1007/s007050050030 Herpes simplex virus type-1 and -2 pathogenesis is restricted by the epidermal basement membrane [Elektronische Daten] [B. S. Weeks, R. S. Ramchandran, J. J. Hopkins, H. M. Friedman] Summary.:  Murine flank scarification with HSV-1 and -2 results in primary lesions at the site of inoculation within three days and lesions at secondary sites within four days. The severity of the infection can be given a numerical value or "score” which is derived from the number and size of these lesions. Using this model, we investigated the role of the epidermal basement membrane in HSV pathogenesis. We exposed murine epidermis to 5×104 plaque forming units of HSV-1 and -2, which by day 8 produced inoculation site (primary site) disease scores of 27 and 12.4 respectively, and secondary site disease scores of 29 and 30 respectively. In contrast, intradermal injection of HSV below the epidermal basement membrane did not cause disease. To determine if the basement membrane restricts HSV spread in vitro, Vero cells were cultured in the lower well of a dual well system. The upper well was separated from the lower well by a filter coated with the artificial basement membrane, matrigel. Addition of virus to the upper well failed to result in either viral accumulation in the lower well or infection of the cells in the lower well. These data suggest that the basement membrane is a barrier to the passage and spread of HSV. 2000 Springer-Verlag/, Wien Weeks B. S. Department of Biology, Adelphi University, Garden City, New York, U.S.A., US aut Ramchandran R. S. Division of Infectious Diseases, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, U.S.A., US aut Hopkins J. J. Department of Biology, Adelphi University, Garden City, New York, U.S.A., US aut Friedman H. M. Division of Infectious Diseases, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, U.S.A., US aut https://doi.org/10.1007/s007050050030 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050050030 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Weeks B. S. Department of Biology, Adelphi University, Garden City, New York, U.S.A., US aut NATIONALLICENCE 700 1- Ramchandran R. S. Division of Infectious Diseases, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, U.S.A., US aut NATIONALLICENCE 700 1- Hopkins J. J. Department of Biology, Adelphi University, Garden City, New York, U.S.A., US aut NATIONALLICENCE 700 1- Friedman H. M. Division of Infectious Diseases, Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, U.S.A., US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer