caa a22 4500 475767446 CHVBK 20180406123610.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s007050070026 doi (NATIONALLICENCE)springer-10.1007/s007050070026 Characterization of a bovine herpesvirus type 4 isolated from the spinal cord of a cow with astasia [Elektronische Daten] [Y. Yamamoto, K. Murakami, Y. Inoshima, T. Nakane, K. Saika, H. Sentsui] Summary.:  A bovine herpesvirus (BHV) strain designated B11-41, was recently isolated from the spinal cord of a cow with astasia. By BHV type specific polymerase chain reaction (PCR), a BHV-4 specific fragment was amplified from the DNA of strain B11-41. The nucleotide sequence of the PCR product revealed high homologies (98.4% and 99.8%) with those of two BHV-4 strains (Movar 33/63 and 86-068). The BamHI restriction endonuclease cleavage patterns of the B11-41 were more similar to those of BHV-4 than those of other types of BHV. BHV-4 is divided into two groups, European and American. The EcoRI, and BamHI, and HindIII restriction endonuclease analysis demonstrated that strain B11-41 was closely related to the American type of BHV-4. It was concluded that B11-41 was identified as a BHV-4 that belongs to the American group. Additionally, the results of this study may indicate that the nervous system is one of the sites of viral latency in natural infection. Springer-Verlag/Wien, 2000 Yamamoto Y. National Institute of Animal Health, Ibaraki, Japan, JP aut Murakami K. National Institute of Animal Health, Ibaraki, Japan, JP aut Inoshima Y. National Institute of Animal Health, Ibaraki, Japan, JP aut Nakane T. Chiba Prefectural Institute of Animal Health, Chiba, Japan, JP aut Saika K. Chiba Prefectural Institute of Animal Health, Chiba, Japan, JP aut Sentsui H. National Institute of Animal Health, Ibaraki, Japan, JP aut Archives of Virology Springer-Verlag 145/11(2000-12-01), 2363-2370 0304-8608 145:11<2363 2000 145 705 https://doi.org/10.1007/s007050070026 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070026 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamamoto Y. National Institute of Animal Health, Ibaraki, Japan, JP aut NATIONALLICENCE 700 1- Murakami K. National Institute of Animal Health, Ibaraki, Japan, JP aut NATIONALLICENCE 700 1- Inoshima Y. National Institute of Animal Health, Ibaraki, Japan, JP aut NATIONALLICENCE 700 1- Nakane T. Chiba Prefectural Institute of Animal Health, Chiba, Japan, JP aut NATIONALLICENCE 700 1- Saika K. Chiba Prefectural Institute of Animal Health, Chiba, Japan, JP aut NATIONALLICENCE 700 1- Sentsui H. National Institute of Animal Health, Ibaraki, Japan, JP aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/11(2000-12-01), 2363-2370 0304-8608 145:11<2363 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer