caa a22 4500 475767497 CHVBK 20180406123610.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s007050070028 doi (NATIONALLICENCE)springer-10.1007/s007050070028 Altered local and systemic spread of movement deficient virus in transgenic tobacco plants expressing the cucumber mosaic virus 3a protein [Elektronische Daten] [A. I. Sanz, M. T. Serra, I. García-Luque] Summary.:  The 3a protein encoded by RNA 3 of cucumber mosaic virus has been identified as the viral cell-to-cell movement protein. The constitutive expression in transgenic tobacco plants of 3a protein from a subgroup I strain was able to complement in trans the short distance movement of a 3a defective CMV mutant belonging to a different taxonomic subgroup. This ability was dependent upon the accumulation levels of the 3a protein in transgenic tobacco plants. However, an initial delay in viral accumulation and spread of the defective virus as compared to the wild type virus was determined in complementation tests. Furthermore, a reduction in disease symptoms as well as a different pattern of systemic viral distribution from those of the wild type virus was detected. These results show that the early events in viral infection affect the long distance spread of the virus. Finally, the wild type virus moved faster in the 3a protein-expressing plants than in control plants, thus indicating that the constitutive expression of the 3a protein favours long-distance viral spread. Springer-Verlag/Wien, 2000 Sanz A. I. Plant Biology Department, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain, ES aut Serra M. T. Plant Biology Department, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain, ES aut García-Luque I. Plant Biology Department, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain, ES aut Archives of Virology Springer-Verlag 145/11(2000-12-01), 2387-2401 0304-8608 145:11<2387 2000 145 705 https://doi.org/10.1007/s007050070028 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070028 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sanz A. I. Plant Biology Department, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain, ES aut NATIONALLICENCE 700 1- Serra M. T. Plant Biology Department, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain, ES aut NATIONALLICENCE 700 1- García-Luque I. Plant Biology Department, Centro de Investigaciones Biológicas (CSIC), Madrid, Spain, ES aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/11(2000-12-01), 2387-2401 0304-8608 145:11<2387 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer