caa a22 4500 475767500 CHVBK 20180406123610.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s007050070027 doi (NATIONALLICENCE)springer-10.1007/s007050070027 EHV-1 glycoprotein D (EHV-1 gD) is required for virus entry and cell-cell fusion, and an EHV-1 gD deletion mutant induces a protective immune response in mice [Elektronische Daten] [H. Csellner, C. Walker, J. E. Wellington, L. E. McLure, D. N. Love, J. M. Whalley] Summary.:  Insertional mutagenesis was used to construct an equine herpesvirus 1 (EHV-1) mutant in which the open reading frame for glycoprotein D was replaced by a lacZ cassette. This gD deletion mutant (ΔgD EHV-1) was unable to infect normally permissive RK cells in culture, but could be propagated in an EHV-1 gD-expressing cell line (RK/gD). Phenotypically complemented ΔgD EHV-1 was able to infect RK cells, but did not spread to form syncytial plaques as seen with wild type EHV-1 or with ΔgD EHV-1 infection of RK/gD cell cultures. Therefore EHV-1 gD is required for virus entry and for cell-cell fusion. The phenotypically complemented ΔgD EHV-1 had very low pathogenicity in a mouse model of EHV-1 respiratory disease, compared to a fully replication-competent EHV-1 reporter virus (lacZ62/63 EHV-1). Intranasal or intramuscular inoculation of mice with ΔgD EHV-1 induced protective immune responses that were similar to those elicited in mice inoculated with lacZ62/63 EHV-1 and greater than those following inoculation with UV-inactivated virus. Springer-Verlag/Wien, 2000 Csellner H. Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia, AU aut Walker C. Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia, AU aut Wellington J. E. Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia, AU aut McLure L. E. Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia, AU aut Love D. N. Department of Veterinary Anatomy and Pathology, University of Sydney, Australia, AU aut Whalley J. M. Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia, AU aut Archives of Virology Springer-Verlag 145/11(2000-12-01), 2371-2385 0304-8608 145:11<2371 2000 145 705 https://doi.org/10.1007/s007050070027 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070027 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Csellner H. Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia, AU aut NATIONALLICENCE 700 1- Walker C. Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia, AU aut NATIONALLICENCE 700 1- Wellington J. E. Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia, AU aut NATIONALLICENCE 700 1- McLure L. E. Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia, AU aut NATIONALLICENCE 700 1- Love D. N. Department of Veterinary Anatomy and Pathology, University of Sydney, Australia, AU aut NATIONALLICENCE 700 1- Whalley J. M. Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, Sydney, Australia, AU aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/11(2000-12-01), 2371-2385 0304-8608 145:11<2371 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer