caa a22 4500 475767608 CHVBK 20180406123610.0 cr unu---uuuuu 170329e20001201xx s 000 0 eng 10.1007/s007050070023 doi (NATIONALLICENCE)springer-10.1007/s007050070023 Zucchini green mottle mosaic virus is a new tobamovirus; comparison of its coat protein gene with that of kyuri green mottle mosaic virus [Elektronische Daten] [K. H. Ryu, B. E. Min, G. S. Choi, S. H. Choi, S. B. Kwon, G. M. Noh, J. Y. Yoon, Y. M. Choi, S. H. Jang, G. P. Lee, K. H. Cho, W. M. Park] Summary.:  A novel virus we call zucchini green mottle mosaic virus (ZGMMV) was isolated from zucchini squash and its properties were determined. The size and shape of its virions, and other properties suggest that the virus is a tobamovirus. The coat protein (CP) genes of ZGMMV and kyuri green mottle mosaic virus (KGMMV), which also infects zucchini squash plants, were cloned and their nucleotides sequences were determined. The CP genes of ZGMMV and KGMMV are composed of 161 amino acid residues, and they share 77.6% amino acid identity. Western blot analysis showed that the two viruses are serologically related but not identical. Comparison of the sequences with those of sixteen other tobamoviruses revealed that the two viruses had much higher identity to cucumber green mottle mosaic virus (CGMMV), another tobamovirus infectious to cucurbit plants, than other tobamoviruses. The nucleotide and amino acid sequences of ZGMMV were from 29.5 to 78.4% and from 29.3 to 77.6% identical, respectively, to those of other tobamoviruses. The predicted virion assembly origins of the two tobamoviruses were located in the CP region of the genomic RNAs, and the predicted secondary structures were more similar to that of CGMMV than those of other tobamoviruses. The seventeen tobamo-viruses could be classified into three main subgroups based on the cphylogenetic tree analysis on the CP gene, and ZGMMV and KGMMV formed a third subgroup together with CGMMV and sunn-hemp mosaic virus (SHMV). These results show that ZGMMV is a previously unknown member of the Tobamovirus genus. Springer-Verlag/Wien, 2000 Ryu K. H. Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, Republic of Korea, KR aut Min B. E. Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, Republic of Korea, KR aut Choi G. S. National Horticultural Research Institute, R.D.A., Suwon, Republic of Korea, KR aut Choi S. H. Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, Republic of Korea, KR aut Kwon S. B. National Horticultural Research Institute, R.D.A., Suwon, Republic of Korea, KR aut Noh G. M. National Horticultural Research Institute, R.D.A., Suwon, Republic of Korea, KR aut Yoon J. Y. Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, Republic of Korea, KR aut Choi Y. M. National Horticultural Research Institute, R.D.A., Suwon, Republic of Korea, KR aut Jang S. H. VaccTech Co. Ltd., Taejon, Republic of Korea, KR aut Lee G. P. Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, Republic of Korea, KR aut Cho K. H. Department of Biology, Seoul Women's University, Seoul, Republic of Korea, KR aut Park W. M. National Research Laboratory for GMO, Graduate School of Biotechnology, Korea University, Seoul, Korea, KR aut Archives of Virology Springer-Verlag 145/11(2000-12-01), 2325-2333 0304-8608 145:11<2325 2000 145 705 https://doi.org/10.1007/s007050070023 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s007050070023 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ryu K. H. Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, Republic of Korea, KR aut NATIONALLICENCE 700 1- Min B. E. Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, Republic of Korea, KR aut NATIONALLICENCE 700 1- Choi G. S. National Horticultural Research Institute, R.D.A., Suwon, Republic of Korea, KR aut NATIONALLICENCE 700 1- Choi S. H. Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, Republic of Korea, KR aut NATIONALLICENCE 700 1- Kwon S. B. National Horticultural Research Institute, R.D.A., Suwon, Republic of Korea, KR aut NATIONALLICENCE 700 1- Noh G. M. National Horticultural Research Institute, R.D.A., Suwon, Republic of Korea, KR aut NATIONALLICENCE 700 1- Yoon J. Y. Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, Republic of Korea, KR aut NATIONALLICENCE 700 1- Choi Y. M. National Horticultural Research Institute, R.D.A., Suwon, Republic of Korea, KR aut NATIONALLICENCE 700 1- Jang S. H. VaccTech Co. Ltd., Taejon, Republic of Korea, KR aut NATIONALLICENCE 700 1- Lee G. P. Plant Virus GenBank, Department of Horticultural Science, Seoul Women's University, Seoul, Republic of Korea, KR aut NATIONALLICENCE 700 1- Cho K. H. Department of Biology, Seoul Women's University, Seoul, Republic of Korea, KR aut NATIONALLICENCE 700 1- Park W. M. National Research Laboratory for GMO, Graduate School of Biotechnology, Korea University, Seoul, Korea, KR aut NATIONALLICENCE 773 0- Archives of Virology Springer-Verlag 145/11(2000-12-01), 2325-2333 0304-8608 145:11<2325 2000 145 705 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer