caa a22 4500 475767624 CHVBK 20180406123610.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/PL00008187 doi (NATIONALLICENCE)springer-10.1007/PL00008187 Identification and spatial distribution of the mRNA encoding an egg envelope component of the Cyprinid zebrafish, Danio rerio , homologous to the mammalian ZP3 (ZPC) [Elektronische Daten] [L. Del Giacco, S. Diani, F. Cotelli] Abstract : Using degenerate reverse transcription polymerase chain reaction (RT-PCR) techniques we have isolated a cDNA encoding a putative component of the zebrafish Danio rerio egg chorion, homologous to the mammalian ZP3 (ZPC). The predicted protein (zfZPC) has a calculated molecular mass of 58.4 kDa and contains a signal peptide (located in the N-terminal region) composed of 11 hydrophobic amino acid residues followed by a signal peptide cleavage site. The zfZPC contains the ZP domain, a characteristic amino acid sequence shared by all ZP proteins of the mammalian zona pellucida and of both amphibian and bird egg envelope components. The zfZPC also exhibits certain unique features including five N-terminal Q-rich tandem repeats presumably involved in the hardening of the chorion after the fertilization of the egg and a long C-terminal tail containing two potential sites of N-linked type glycosylation. RT-PCR and in situ hybridization revealed a restricted pattern of tissue distribution: the gene encoding zfZPC is transcribed only in the growing oocyte of sexually mature female fish. Springer-Verlag Berlin Heidelberg, 2000 Key words Fish nationallicence Egg envelope nationallicence ZP domain nationallicence N-terminal repeats nationallicence Del Giacco L. Dipartimento di Biologia, Università di Milano, via Celoria, 26, I-20133 Milan, Italy e-mail: franco.cotelli@unimi.it, IT aut Diani S. Dipartimento di Biologia, Università di Milano, via Celoria, 26, I-20133 Milan, Italy e-mail: franco.cotelli@unimi.it, IT aut Cotelli F. Dipartimento di Biologia, Università di Milano, via Celoria, 26, I-20133 Milan, Italy e-mail: franco.cotelli@unimi.it, IT aut https://doi.org/10.1007/PL00008187 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00008187 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Del Giacco L. Dipartimento di Biologia, Università di Milano, via Celoria, 26, I-20133 Milan, Italy e-mail: franco.cotelli@unimi.it, IT aut NATIONALLICENCE 700 1- Diani S. Dipartimento di Biologia, Università di Milano, via Celoria, 26, I-20133 Milan, Italy e-mail: franco.cotelli@unimi.it, IT aut NATIONALLICENCE 700 1- Cotelli F. Dipartimento di Biologia, Università di Milano, via Celoria, 26, I-20133 Milan, Italy e-mail: franco.cotelli@unimi.it, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer