caa a22 4500 475767667 CHVBK 20180406123610.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/PL00008181 doi (NATIONALLICENCE)springer-10.1007/PL00008181 Characterization of a hemichordate fork head/HNF-3 gene expression [Elektronische Daten] [S. Taguchi, K. Tagawa, T. Humphreys, A. Nishino, N. Satoh, Y. Harada] Abstract : Based on anatomical and developmental similarities, hemichordates are thought to be most closely related to chordates. However, so far very few developmental genes have been characterized from hemichordates. To gain molecular insight into the developmental mechanisms involved in the origin and evolution of chordates, we investigated the expression of a fork head/HNF-3 (PfHNF3) gene in the acorn worm embryo. Chordate fork head genes are implicated in the formation of endoderm, notochord and floor plate. We found that a PfHNF3 transcript was first detected at the early blastula stage; the signal of in situ hybridization was found in the vegetal plate cells, invaginating endoderm and then in the archenteron. By the late gastrula and into the early tornaria larva stages, an intense signal remained in the anterior region of the archenteron, while the expression in the other regions of archenteron decreased. The intense signal was retained in the pharynx of the tornaria larva. A comparison of the pattern of PfHNF3 with that of HNF-3 genes of sea urchin, ascidian, amphioxus and vertebrate suggests a possible acquisition of new functions of the gene during deuterostome evolution. Springer-Verlag Berlin Heidelberg, 2000 Key words Hemichordates nationallicence Deuterostomes nationallicence fork head/HNF-3 gene nationallicence Vegetal plate cells nationallicence Archenteron nationallicence Taguchi S. Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan E-mail: taguchi@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut Tagawa K. Kewalo Marine Laboratory, Pacific Biomedical Research Center, University of Hawaii, 41 Ahui Street, Honolulu, HI 96813, USA, US aut Humphreys T. Kewalo Marine Laboratory, Pacific Biomedical Research Center, University of Hawaii, 41 Ahui Street, Honolulu, HI 96813, USA, US aut Nishino A. Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan E-mail: taguchi@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut Satoh N. Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan E-mail: taguchi@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut Harada Y. Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan E-mail: taguchi@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut https://doi.org/10.1007/PL00008181 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00008181 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taguchi S. Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan E-mail: taguchi@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut NATIONALLICENCE 700 1- Tagawa K. Kewalo Marine Laboratory, Pacific Biomedical Research Center, University of Hawaii, 41 Ahui Street, Honolulu, HI 96813, USA, US aut NATIONALLICENCE 700 1- Humphreys T. Kewalo Marine Laboratory, Pacific Biomedical Research Center, University of Hawaii, 41 Ahui Street, Honolulu, HI 96813, USA, US aut NATIONALLICENCE 700 1- Nishino A. Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan E-mail: taguchi@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut NATIONALLICENCE 700 1- Satoh N. Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan E-mail: taguchi@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut NATIONALLICENCE 700 1- Harada Y. Department of Zoology, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan E-mail: taguchi@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer