caa a22 4500 475767683 CHVBK 20180406123610.0 cr unu---uuuuu 170329e20000101xx s 000 0 eng 10.1007/PL00008185 doi (NATIONALLICENCE)springer-10.1007/PL00008185 An in situ hybridization screen for the rapid isolation of differentially expressed genes [Elektronische Daten] [T. Henrich, J. Wittbrodt] Abstract : To rapidly isolate genes specifically expressed during medaka development we generated a cDNA library enriched for genes expressed in the head region of the developing embryo. Clones were spotted on filters automatically and preselected for abundantly expressed genes by hybridizing them with a probe derived from RNA of undifferentiated totipotent cells. Of the nonhybridizing clones 153 were chosen randomly and further analyzed by whole-mount in situ hybridization. There were 67 selected clones differentially expressed in the developing embryos, and 48 of these were expressed in the developing head. Differentially expressed genes were either of novel type or showed homology to known genes containing DNA binding motifs or to putative housekeeping genes. Springer-Verlag Berlin Heidelberg, 2000 Key words Random picking nationallicence In situ hybridization nationallicence Medaka fish nationallicence Development nationallicence ATPase nationallicence Malate dehydrogenase nationallicence Henrich T. Max Planck Institute for Biophysical Chemistry, Am Fassberg, D-37077 Göttingen, Germany, DE aut Wittbrodt J. Max Planck Institute for Biophysical Chemistry, Am Fassberg, D-37077 Göttingen, Germany, DE aut https://doi.org/10.1007/PL00008185 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00008185 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Henrich T. Max Planck Institute for Biophysical Chemistry, Am Fassberg, D-37077 Göttingen, Germany, DE aut NATIONALLICENCE 700 1- Wittbrodt J. Max Planck Institute for Biophysical Chemistry, Am Fassberg, D-37077 Göttingen, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer