caa a22 4500 475767756 CHVBK 20180406123611.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s004270050318 doi (NATIONALLICENCE)springer-10.1007/s004270050318 Nervous system dynamics during fragmentation and regeneration in Enchytraeus japonensis (Oligochaeta, Annelida) [Elektronische Daten] [C. Yoshida-Noro, M. Myohara, F. Kobari, S. Tochinai] Abstract : Enchytraeus japonensis is a small terrestrial oligochaete which primarily reproduces asexually by fragmentation and regeneration. In order to introduce a molecular approach to the study of regeneration we developed a whole-mount immunostaining procedure for the worm. Using an antibody directed against acetylated tubulin in conjunction with confocal laser-scanning microscopy, we succeeded in clarifying the three- dimensional structure of the entire nervous system in the full-grown worm and its dynamics during the fragmentation and regeneration process. In addition, we examined the expression of neurotransmitters and neuropeptides in the worm using a fluorescently-labeled antagonist and various antibodies. In particular, we found two circumferential structures in the body wall muscle of each segment that react strongly with α-bungarotoxin, an antagonist of nicotinic acetylcholine receptors, and detected nerve fibers just underneath these structures. During the fragmentation process, the circular body wall muscles contract near one of these circumferential structures in the middle of the segment, which causes constriction and results in fission of the body. This α-bungarotoxin-positive structure was designated the neuromuscular junction of the circular muscle. During the regeneration process nerve fibers grow from the remaining ventral nerve cord and gradually form networks in both the anterior and posterior regeneration buds. The growing fibers extend to the prostomium (a sensory organ) at the anterior end prior to connecting to the presumptive brain rudiment. A neural network appears around the pygidium, and this is followed by growth of the body at the posterior end. The nervous system appears to play an important role in both anterior and posterior regeneration. Springer-Verlag Berlin Heidelberg, 2000 Key words Regeneration nationallicence Fragmentation nationallicence Nervous system nationallicence Annelid nationallicence Confocal microscopy nationallicence Yoshida-Noro C. Brainway Group, Brain Science Institute, The Institute of Physical and Chemical Research (RIKEN), Wako 351-0198, Japan, JP aut Myohara M. Department of Insect Genetics and Breeding, National Institute of Sericultural and Entomological Science, Tsukuba 305-8634, Japan, JP aut Kobari F. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan, JP aut Tochinai S. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan, JP aut https://doi.org/10.1007/s004270050318 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270050318 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoshida-Noro C. Brainway Group, Brain Science Institute, The Institute of Physical and Chemical Research (RIKEN), Wako 351-0198, Japan, JP aut NATIONALLICENCE 700 1- Myohara M. Department of Insect Genetics and Breeding, National Institute of Sericultural and Entomological Science, Tsukuba 305-8634, Japan, JP aut NATIONALLICENCE 700 1- Kobari F. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan, JP aut NATIONALLICENCE 700 1- Tochinai S. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer