caa a22 4500 475767764 CHVBK 20180406123611.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/PL00008189 doi (NATIONALLICENCE)springer-10.1007/PL00008189 Analysis of four embryo-specific mutants in Zea mays reveals that incomplete radial organization of the proembryo interferes with subsequent development [Elektronische Daten] [R. Elster, P. Bommert, W.F. Sheridan, W. Werr] Abstract : Using confocal laser scanning microscopy we have characterized early and intermediate stages of maize wild-type embryogenesis and compared to mutant development of four different embryo-specific mutations, emb*-8518, emb*-8521, emb*-8537, and emb*-8542. Confocal laser scanning microscopy is well suited to study embryo development in maize in a nondisruptive manner from shortly after fertilization to late stages in embryogenesis. The analysis of the mutant morphology indicated that two of the recessive mutations, emb*-8518 and emb*-8521,cause an early developmental arrest in the proembryo/early transition stage: mutant embryos are unable to enter the morphogenetic phase of embryogenesis. In contrast, homozygous emb*-8537, and emb*-8542 embryos progress at least to the coleoptilar stage and sometimes establish a functional shoot meristem that can determine leaf primordia. The morphological characterization of mutants was confirmed by analysis of the expression pattern of three different marker genes: Lipid transfer protein 2, Zea mays Outer Cell Layer 1, and Knotted 1. Our data indicate that both emb*-8518 and emb*-8521 mutant embryos are impaired in restriction of ZmOCL1 transcripts to the embryonic protoderm and therefore fail to establish a normal radial organization. In contrast, emb*-8537 and emb*-8542 embryos exhibit the wild-type pattern and proceed in development to the formation of a shoot apical meristem and the establishment of bilateral symmetry. Springer-Verlag Berlin Heidelberg, 2000 Key words Zea mays nationallicence Embryo-specific nationallicence Protoderm nationallicence Shoot meristem nationallicence Radial organization nationallicence Elster R. Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Cologne, Germany E-mail: w.werr@uni-koeln.de Tel.: +49-221-4702619, Fax: +49-221-4705164, DE aut Bommert P. Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Cologne, Germany E-mail: w.werr@uni-koeln.de Tel.: +49-221-4702619, Fax: +49-221-4705164, DE aut Sheridan W.F. Department of Biology, University of North Dakota, Grand Forks, ND 58202, USA, US aut Werr W. Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Cologne, Germany E-mail: w.werr@uni-koeln.de Tel.: +49-221-4702619, Fax: +49-221-4705164, DE aut https://doi.org/10.1007/PL00008189 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/PL00008189 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Elster R. Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Cologne, Germany E-mail: w.werr@uni-koeln.de Tel.: +49-221-4702619, Fax: +49-221-4705164, DE aut NATIONALLICENCE 700 1- Bommert P. Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Cologne, Germany E-mail: w.werr@uni-koeln.de Tel.: +49-221-4702619, Fax: +49-221-4705164, DE aut NATIONALLICENCE 700 1- Sheridan W.F. Department of Biology, University of North Dakota, Grand Forks, ND 58202, USA, US aut NATIONALLICENCE 700 1- Werr W. Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Cologne, Germany E-mail: w.werr@uni-koeln.de Tel.: +49-221-4702619, Fax: +49-221-4705164, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer