caa a22 4500 475767845 CHVBK 20180406123611.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004270000110 doi (NATIONALLICENCE)springer-10.1007/s004270000110 A versatile vector set for animal transgenesis [Elektronische Daten] [Carsten Horn, E. A. Wimmer] Abstract : Genetic manipulation of a series of diverged arthropods is a highly desirable goal for a better understanding of developmental and evolutionary processes. A major obstacle so far has been the difficulty in obtaining marker genes that allow easy and reliable identification of transgenic animals. Here, we present a versatile vector set for germline transformation based on the promiscuous transposons mariner, Hermes and piggyBac. Into these vectors, we introduced a potentially universal marker system that is comprised of an artificial promoter containing three Pax-6 homodimer binding sites. This promoter drives strong expression of spectral variants of the enhanced green fluorescent protein (EGFP) in larval, pupal, and adult photoreceptors. Using special filter sets, the yellow (EYFP) and cyan (ECFP) variant are fully distinguishable and therefore represent a separable pair of markers. Furthermore, we adapted a simple plasmid-based transposition assay system to enable quick functional tests of our vectors in different arthropod species before employing them in more laborious germline transformation experiments. Using this system we demonstrate that our vectors transpose in both Drosophila melanogaster and Drosophila virilis. Springer-Verlag Berlin Heidelberg, 2000 Keywords Green fluorescent protein nationallicence Pax-6 nationallicence Transformation marker nationallicence Transposable elements nationallicence Transposon mutagenesis nationallicence Horn Carsten Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstrasse 30 NWI, 95447 Bayreuth, Germany e-mail: ernst.wimmer@uni-bayreuth.de Tel.: +49-921-555813, Fax: +49-921-552710, DE aut Wimmer E. A. Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstrasse 30 NWI, 95447 Bayreuth, Germany e-mail: ernst.wimmer@uni-bayreuth.de Tel.: +49-921-555813, Fax: +49-921-552710, DE aut https://doi.org/10.1007/s004270000110 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270000110 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Horn Carsten Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstrasse 30 NWI, 95447 Bayreuth, Germany e-mail: ernst.wimmer@uni-bayreuth.de Tel.: +49-921-555813, Fax: +49-921-552710, DE aut NATIONALLICENCE 700 1- Wimmer E. A. Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstrasse 30 NWI, 95447 Bayreuth, Germany e-mail: ernst.wimmer@uni-bayreuth.de Tel.: +49-921-555813, Fax: +49-921-552710, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer