caa a22 4500 475767853 CHVBK 20180406123611.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004270000103 doi (NATIONALLICENCE)springer-10.1007/s004270000103 Molecular and biological characterization of a zonula occludens-1 homologue in Hydra vulgaris, named HZO-1 [Elektronische Daten] [Kaiyin Fei, L. Yan, Jinsong Zhang, M. P. Sarras Jr.] Abstract : Zonula occludens-1 (ZO-1) is one of the earliest identified molecular components of tight junctions. Sequence analysis has placed ZO-1 into the broader membrane-associated guanylate kinase (MAGUK) protein family that contains such diverse members as postsynaptic density 95 (PSD-95), Drosophila discs large tumor suppressor gene product (dlg-A), p55, and TamA. Studies in both vertebrates and invertebrates have established that the MAGUK family is involved in a wide variety of cellular functions. These functions involve the regulation of such cellular processes as: (1) tight junction formation, (2) cell proliferation, (3) cell differentiation, and (4) neuronal synapse transmission. Extending these studies, we report the presence of a ZO-1 homologue in Hydra vulgaris, a member of the Cnidaria, the second oldest phylum of the animal kingdom. Hydra ZO-1 (HZO-1) is encoded by a single messenger RNA (mRNA) of approximately 6.0 kb that contains an open reading frame of 5,085 bp. The 191 kDa predicted protein consists of a characteristic MAGUK domain structure, including three PSD-95/SAP90, discs-large, ZO-1 (PDZ) domains, a src homology (SH3) domain, and a guanylate kinase (GUK) domain. Western blot analysis using an antibody generated from a synthetic peptide designed from the HZO-1 sequence confirmed the presence of a Hydra protein of the appropriate mass. While whole mount in situ hybridization determined that HZO-1 mRNA was expressed along the entire longitudinal axis of Hydra, cross-sectional analysis established that HZO-1 mRNA expression was restricted to the ectoderm or outer cell layer of the organism's epithelial bilayer. Consistent with this mRNA expression pattern, immunofluorescence studies localized HZO-1 protein to the apical plasma membrane of ectodermal cells. It is unclear what role HZ0-1 has in the cellular physiology of Hydra; however, immunolocalization studies indicate a conserved plasma membrane-associated function(s), as reported for its counterparts in other invertebrate and vertebrate species. These studies establish that the MAGUK family of proteins with a membrane-associated function arose early during metazoan evolution, even before the divergence of protostomes and deuterostomes. Springer-Verlag Berlin Heidelberg, 2000 Keywords Hydra nationallicence ZO-1 nationallicence Plasma membrane-associated protein nationallicence MAGUK nationallicence Fei Kaiyin Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kuma.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut Yan L. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kuma.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut Zhang Jinsong Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kuma.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut Sarras Jr. M. P. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kuma.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut https://doi.org/10.1007/s004270000103 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270000103 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fei Kaiyin Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kuma.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut NATIONALLICENCE 700 1- Yan L. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kuma.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut NATIONALLICENCE 700 1- Zhang Jinsong Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kuma.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut NATIONALLICENCE 700 1- Sarras Jr M. P. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kuma.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer