caa a22 4500 475767861 CHVBK 20180406123611.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004270000102 doi (NATIONALLICENCE)springer-10.1007/s004270000102 CAAT sites are required for the activation of the H. pulcherrimus Ars gene by Otx [Elektronische Daten] [Takae Kiyama, Kaori Sasai, Kazuko Takata, Keiko Mitsunaga-Nakatsubo, Hiraku Shimada, K. Akasaka] Abstract : A product of sea urchin homologues of the Drosophila orthodenticle gene, HpOtxL has been implicated as a transcription activator of the aboral ectoderm-specific arylsulfatase (Ars) gene during early development of the sea urchin embryo. Using an in vivo transactivation system, we present evidence that HpOtxL activates the target gene by interacting with co-factors. Otx binding sites alone have little effect on the activity of an Ars promoter, but when both Otx binding sites and CAAT sequences are present in the enhancer region of Ars, the fragment shows a high enhancer activity. A gel mobility shift assay reveals that a nuclear protein binds to the CAAT sequences present near the Otx binding sites in the enhancer region of Ars. The activation domain of HpOtxL resides in the C terminal region between amino acids 218 and 238. The N-terminal region is responsible for the enhancement of transactivation of the Ars promoter, although the region itself does not function as an activation domain. HpOtxE, which possesses an N-terminal region different from HpOtxL, does not activate the Ars promoter even in the presence of CAAT sequences. Together with previous findings, our results suggest that Otx regulates different genes by interacting with different co-factors in sea urchin development. Springer-Verlag Berlin Heidelberg, 2000 Keywords Sea urchin nationallicence Otx nationallicence Transactivation nationallicence Kiyama Takae Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut Sasai Kaori Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut Takata Kazuko Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut Mitsunaga-Nakatsubo Keiko Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut Shimada Hiraku Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut Akasaka K. Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut https://doi.org/10.1007/s004270000102 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270000102 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kiyama Takae Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut NATIONALLICENCE 700 1- Sasai Kaori Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut NATIONALLICENCE 700 1- Takata Kazuko Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut NATIONALLICENCE 700 1- Mitsunaga-Nakatsubo Keiko Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut NATIONALLICENCE 700 1- Shimada Hiraku Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut NATIONALLICENCE 700 1- Akasaka K. Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima 739-8526, Japan e-mail: koji@hiroshima-u.ac.jp Tel.: +81-824-24-7447, Fax: +81-824-24-0733, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer