caa a22 4500 475767896 CHVBK 20180406123611.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004270000109 doi (NATIONALLICENCE)springer-10.1007/s004270000109 On the conservation of function of the Drosophila Fat facets deubiquitinating enzyme and Fam, its mouse homolog [Elektronische Daten] [X. Chen, Erin Overstreet, Stephen A. Wood, J. A. Fischer] Abstract : Fat facets is a Drosophila deubiquitinating enzyme required for eye development and early embryogenesis. Genetic evidence suggests that Fat facets deubiquitinates and thereby prevents the proteasomal degradation of specific substrates. The Drosophila Liquid facets protein is implicated as the critical substrate of Fat facets in the eye. A mouse homolog of Fat facets, called Fam, has been identified. The results of biochemical experiments implicate two different proteins, Af-6 and β-catenin, as substrates for Fam. Here, the functional relationship between Fat facets and Fam is explored. We show that Fam can substitute for Fat facets in all of its essential functions in Drosophila. In addition, we tested the hypothesis that Canoe and Armadillo, the Drosophila homologs of Af-6 and β-catenin, respectively, are important substrates for Fat facets in the Drosophila eye. We found no genetic evidence to support a role for either Canoe or Armadillo in the essential Fat facets pathways in Drosophila eye development. The significance of these results is discussed in light of the biochemical experiments that suggest that Af-6 and β-catenin are substrates of Fam. Springer-Verlag Berlin Heidelberg, 2000 Keywords Fat facets nationallicence Deubiquitinating enzyme nationallicence Fam nationallicence Af-6 nationallicence β-Catenin nationallicence Chen X. Section of Molecular Cell and Developmental Biology and The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Moffett Molecular Biology Building, 2500 Speedway, Austin, TX 78712, USA e-mail: jaf@mail.utexas.edu Tel.: +1-512-4715831, Fax: +1-512-2323432, US aut Overstreet Erin Section of Molecular Cell and Developmental Biology and The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Moffett Molecular Biology Building, 2500 Speedway, Austin, TX 78712, USA e-mail: jaf@mail.utexas.edu Tel.: +1-512-4715831, Fax: +1-512-2323432, US aut Wood Stephen A. Department of Biochemistry, University of Adelaide, Adelaide SA 5005, Australia, AU aut Fischer J. A. Section of Molecular Cell and Developmental Biology and The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Moffett Molecular Biology Building, 2500 Speedway, Austin, TX 78712, USA e-mail: jaf@mail.utexas.edu Tel.: +1-512-4715831, Fax: +1-512-2323432, US aut https://doi.org/10.1007/s004270000109 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270000109 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chen X. Section of Molecular Cell and Developmental Biology and The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Moffett Molecular Biology Building, 2500 Speedway, Austin, TX 78712, USA e-mail: jaf@mail.utexas.edu Tel.: +1-512-4715831, Fax: +1-512-2323432, US aut NATIONALLICENCE 700 1- Overstreet Erin Section of Molecular Cell and Developmental Biology and The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Moffett Molecular Biology Building, 2500 Speedway, Austin, TX 78712, USA e-mail: jaf@mail.utexas.edu Tel.: +1-512-4715831, Fax: +1-512-2323432, US aut NATIONALLICENCE 700 1- Wood Stephen A. Department of Biochemistry, University of Adelaide, Adelaide SA 5005, Australia, AU aut NATIONALLICENCE 700 1- Fischer J. A. Section of Molecular Cell and Developmental Biology and The Institute for Cellular and Molecular Biology, The University of Texas at Austin, Moffett Molecular Biology Building, 2500 Speedway, Austin, TX 78712, USA e-mail: jaf@mail.utexas.edu Tel.: +1-512-4715831, Fax: +1-512-2323432, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer