caa a22 4500 47576790X CHVBK 20180406123611.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004270000105 doi (NATIONALLICENCE)springer-10.1007/s004270000105 Expression and evolutionary conservation of nanos- related genes in Hydra [Elektronische Daten] [Kazufumi Mochizuki, Hiroko Sano, Satoru Kobayashi, Chiemi Nishimiya-Fujisawa, T. Fujisawa] Abstract : The Drosophila gene nanos encodes two particular zinc finger motifs which are also found in germline-associated factors from nematodes to vertebrates. We cloned two nanos (nos)-related genes, Cnnos1 and Cnnos2 from Hydra magnipapillata. Using whole-mount in situ hybridization, the expression of Cnnos1 and Cnnos2 was examined. Cnnos1 was specifically expressed in multipotent stem cells and germline cells, but not in somatic cells. Cnnos2 was weakly expressed in germline cells and more specifically in the endoderm of the hypostome where it appears to be involved in head morphogenesis. In addition to structural conservation in the zinc finger domain of nanos-related genes, functional conservation of Cnnos1 was also demonstrated by the finding that a Cnnos1 transgene can partially rescue the nos RC phenotype that is defective in the egg production of Drosophila. Thus, the function of nanos-related genes in the germline appears to be well conserved from primitive to highly evolved metazoans. Springer-Verlag Berlin Heidelberg, 2000 Keywords Hydra nationallicence nanos-related genes nationallicence Expression in germ cells and epithelial cells nationallicence Evolutionary conservation nationallicence Mochizuki Kazufumi Department of Genetics, The Graduate University for Advanced Studies, Mishima 411-8540, Japan, JP aut Sano Hiroko Institute of Biological Sciences, University of Tsukuba, Tsukuba 305-8572, Japan, JP aut Kobayashi Satoru Institute of Biological Sciences, Center for Tsukuba Advanced Research Alliance, and Gene Experiment Center, University of Tsukuba, Tsukuba 305-8572, Japan, JP aut Nishimiya-Fujisawa Chiemi Department of Developmental Genetics, National Institute of Genetics, Mishima 411-8540, Japan, JP aut Fujisawa T. Department of Genetics, The Graduate University for Advanced Studies and Department of Developmental Genetics, National Institute of Genetics, Mishima 411-8540, Japan e-mail: tfujisaw@lab.nig.ac.jp, JP aut https://doi.org/10.1007/s004270000105 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270000105 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mochizuki Kazufumi Department of Genetics, The Graduate University for Advanced Studies, Mishima 411-8540, Japan, JP aut NATIONALLICENCE 700 1- Sano Hiroko Institute of Biological Sciences, University of Tsukuba, Tsukuba 305-8572, Japan, JP aut NATIONALLICENCE 700 1- Kobayashi Satoru Institute of Biological Sciences, Center for Tsukuba Advanced Research Alliance, and Gene Experiment Center, University of Tsukuba, Tsukuba 305-8572, Japan, JP aut NATIONALLICENCE 700 1- Nishimiya-Fujisawa Chiemi Department of Developmental Genetics, National Institute of Genetics, Mishima 411-8540, Japan, JP aut NATIONALLICENCE 700 1- Fujisawa T. Department of Genetics, The Graduate University for Advanced Studies and Department of Developmental Genetics, National Institute of Genetics, Mishima 411-8540, Japan e-mail: tfujisaw@lab.nig.ac.jp, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer