caa a22 4500 475767918 CHVBK 20180406123611.0 cr unu---uuuuu 170329e20001101xx s 000 0 eng 10.1007/s004270000106 doi (NATIONALLICENCE)springer-10.1007/s004270000106 Origin of the paired domain [Elektronische Daten] [Rainer Breitling, J.-K. Gerber] Abstract : Pax proteins play a diverse role in early animal development and contain the characteristic paired domain, consisting of two conserved helix-turn-helix motifs. In many Pax proteins the paired domain is fused to a second DNA binding domain of the paired-like homeobox family. By amino acid sequence alignments, secondary structure prediction, 3D-structure comparison, and phylogenetic reconstruction, we analyzed the relationship between Pax proteins and members of the Tc1 family of transposases, which possibly share a common ancestor with Pax proteins. We suggest that the DNA binding domain of an ancestral transposase (proto-Pax transposase) was fused to a homeodomain shortly after the emergence of metazoans about one billion years ago. Using the transposase sequences as an outgroup we reexamined the early evolution of the Pax proteins. Our novel evolutionary scenario features a single homeobox capturing event and an early duplication of Pax genes before the divergence of porifera, indicating a more diverse role of Pax proteins in primitive animals than previously expected. Springer-Verlag Berlin Heidelberg, 2000 Keywords Pax protein nationallicence Paired domain nationallicence Homeobox nationallicence Transposase nationallicence Evolution nationallicence Breitling Rainer GSF-National Research Center for Environment and Health, Institute of Experimental Genetics, 85764 Neuherberg, Germany e-mail: gerber@gsf.de Tel.: +49-89-31873229, Fax: +49-89-31873225, DE aut Gerber J.-K GSF-National Research Center for Environment and Health, Institute of Experimental Genetics, 85764 Neuherberg, Germany e-mail: gerber@gsf.de Tel.: +49-89-31873229, Fax: +49-89-31873225, DE aut https://doi.org/10.1007/s004270000106 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270000106 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Breitling Rainer GSF-National Research Center for Environment and Health, Institute of Experimental Genetics, 85764 Neuherberg, Germany e-mail: gerber@gsf.de Tel.: +49-89-31873229, Fax: +49-89-31873225, DE aut NATIONALLICENCE 700 1- Gerber J.-K GSF-National Research Center for Environment and Health, Institute of Experimental Genetics, 85764 Neuherberg, Germany e-mail: gerber@gsf.de Tel.: +49-89-31873229, Fax: +49-89-31873225, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer