caa a22 4500 475767977 CHVBK 20180406123611.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s004270000082 doi (NATIONALLICENCE)springer-10.1007/s004270000082 Two engrailed -related genes in the cockroach: cloning, phylogenetic analysis, expression and isolation of splice variants [Elektronische Daten] [Bruno Marie, J. P. Bacon] Abstract  : engrailed-related genes have been isolated in numerous taxa. Within the insects, some species have a single engrailed-related gene whilst others have two copies, raising the question of when and how often gene duplications have occurred. Here we report the cloning, in the cockroach Periplaneta americana, of two engrailed-related genes Pa-en1 and Pa-en2. By comparing conserved domains and by carrying out a phylogenetic analysis, we conclude that these two genes are likely to be the product of a recent duplication in the cockroach lineage. Pa-en1 and Pa-en2 are co-expressed during early embryogenesis and their segmental pattern of expression appears in an anterior-posterior progression. We have also isolated potential splice variants of Pa-en2 which lack some regulatory domains. The roles these splice variants may play in regulating developmental processes are discussed. Springer-Verlag Berlin Heidelberg, 2000 Key words Evolution nationallicence Insect nationallicence Periplaneta americana nationallicence Segmentation nationallicence Marie Bruno Sussex Centre for Neuroscience, School of Biological Sciences, University of Sussex, Brighton, BN1 9QG, UK e-mail: j.p.bacon@sussex.ac.uk Tel.: +44-1273-678489, Fax: +44-1273-678535, GB aut Bacon J. P. Sussex Centre for Neuroscience, School of Biological Sciences, University of Sussex, Brighton, BN1 9QG, UK e-mail: j.p.bacon@sussex.ac.uk Tel.: +44-1273-678489, Fax: +44-1273-678535, GB aut https://doi.org/10.1007/s004270000082 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270000082 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Marie Bruno Sussex Centre for Neuroscience, School of Biological Sciences, University of Sussex, Brighton, BN1 9QG, UK e-mail: j.p.bacon@sussex.ac.uk Tel.: +44-1273-678489, Fax: +44-1273-678535, GB aut NATIONALLICENCE 700 1- Bacon J. P. Sussex Centre for Neuroscience, School of Biological Sciences, University of Sussex, Brighton, BN1 9QG, UK e-mail: j.p.bacon@sussex.ac.uk Tel.: +44-1273-678489, Fax: +44-1273-678535, GB aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer