caa a22 4500 475768051 CHVBK 20180406123611.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s004270050024 doi (NATIONALLICENCE)springer-10.1007/s004270050024 Expression patterns of musashi homologs of the ascidians, Halocynthia roretzi and Ciona intestinalis [Elektronische Daten] [T. Kawashima, A. R. Murakami, M. Ogasawara, K. J. Tanaka, R. Isoda, Y. Sasakura, T. Nishikata, H. Okano, K. W. Makabe] Abstract : The gene family encoding RNA-binding proteins includes important regulators involved in the neurogenesis in both protostomes and deuterostomes. We isolated cDNAs of the ascidian homolog of one of the RNA-binding proteins, MUSASHI, from Halocynthia roretzi and Ciona intestinalis. The predicted amino acid sequences contained two RNA-recognition and RNA-binding motifs in the N-terminus and an ascidian-specific YG-rich domain in the C-terminus. Maternal transcripts of musashi were ubiquitous in early cleavage-stage embryos. Ascidian musashi had three domains of zygotic expression: the brain, nerve cord, and mesenchyma. The temporal order of the onset in these domains was highly divergent between the two species of ascidian examined. Springer-Verlag Berlin Heidelberg, 2000 Key words Ascidian nationallicence Musashi nationallicence RNA-binding protein nationallicence Kawashima T. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan E-mail: kwmakabe@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut Murakami A. R. Department of Biology, Faculty of Science, Konan University, Kobe, Hyogo 658-8501, Japan, JP aut Ogasawara M. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan E-mail: kwmakabe@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut Tanaka K. J. Department of Biology, Faculty of Science, Konan University, Kobe, Hyogo 658-8501, Japan, JP aut Isoda R. Department of Biology, Faculty of Science, Konan University, Kobe, Hyogo 658-8501, Japan, JP aut Sasakura Y. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan E-mail: kwmakabe@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut Nishikata T. Department of Biology, Faculty of Science, Konan University, Kobe, Hyogo 658-8501, Japan, JP aut Okano H. Department of Neuroanatomy, Biomedical Reseach Center, Osaka University Medical School, Suita, Osaka 565-0871, Japan, JP aut Makabe K. W. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan E-mail: kwmakabe@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut https://doi.org/10.1007/s004270050024 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270050024 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kawashima T. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan E-mail: kwmakabe@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut NATIONALLICENCE 700 1- Murakami A. R. Department of Biology, Faculty of Science, Konan University, Kobe, Hyogo 658-8501, Japan, JP aut NATIONALLICENCE 700 1- Ogasawara M. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan E-mail: kwmakabe@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut NATIONALLICENCE 700 1- Tanaka K. J. Department of Biology, Faculty of Science, Konan University, Kobe, Hyogo 658-8501, Japan, JP aut NATIONALLICENCE 700 1- Isoda R. Department of Biology, Faculty of Science, Konan University, Kobe, Hyogo 658-8501, Japan, JP aut NATIONALLICENCE 700 1- Sasakura Y. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan E-mail: kwmakabe@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut NATIONALLICENCE 700 1- Nishikata T. Department of Biology, Faculty of Science, Konan University, Kobe, Hyogo 658-8501, Japan, JP aut NATIONALLICENCE 700 1- Okano H. Department of Neuroanatomy, Biomedical Reseach Center, Osaka University Medical School, Suita, Osaka 565-0871, Japan, JP aut NATIONALLICENCE 700 1- Makabe K. W. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan E-mail: kwmakabe@ascidian.zool.kyoto-u.ac.jp Fax: +81-75-705-1113, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer