caa a22 4500 475768132 CHVBK 20180406123612.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004270000090 doi (NATIONALLICENCE)springer-10.1007/s004270000090 Evolution of distinct expression patterns for engrailed paralogues in higher crustaceans (Malacostraca) [Elektronische Daten] [A. Ahzhanov, T. C. Kaufman] Abstract : The segment-polarity gene engrailed of Drosophila melanogaster and its homologues in other arthropods possess a highly conserved expression domain in the posterior portion of each segment. We report here that the two pan-specific antibodies, Mab4D9 and Mab4F11, reveal strikingly different accumulation patterns in both of the malacostracan crustaceans Porcellio scaber (Isopoda) and Procambarus clarkii (Decapoda), compared with insects. The signal detected with Mab4D9 resides in the posterior part of each segment, including the appendages, the ventral and lateral sides of the trunk and the CNS. However, Mab4F11 reveals a signal only in small groups of neurons in the CNS and PNS, primarily localized in the pereon. We observe similar Mab4D9 and Mab4F11 patterns in the crayfish P. clarkii, except that no Mab4F11 signal is detected in the pleon. To address the possibility of multiple engrailed paralogues, we cloned partial cDNAs of two engrailed genes, Ps-en1 and Ps-en2, from P. scaber, and studied their expression patterns using whole-mount in situ hybridization. Although the Ps-en1 and Ps-en2 patterns are comparable in early development, they become distinct in late embryogenesis. Ps-en1 is expressed in the CNS, where Mab4F11 stains, but also accumulates in the epidermis. In contrast, Ps-en2 is expressed in the lateral aspect and limbs of all segments. Phylogenetic analysis of en sequences from crustaceans and insects suggests that the two en genes from the apterygote insect Thermobia domestica (Thysanura) may be related to en1 and en2 of higher crustaceans. Springer-Verlag Berlin Heidelberg, 2000 Key words Crustacean nationallicence Insect nationallicence engrailed nationallicence Mab4D9 nationallicence Mab4F11 nationallicence Ahzhanov A. Howard Hughes Medical Institute, Department of Biology, Indiana University, Bloomington, IN 47405, USA e-mail: kaufman@bio.indiana.edu Tel +1-812-8553033, Fax +1-812-8552577, US aut Kaufman T. C. Howard Hughes Medical Institute, Department of Biology, Indiana University, Bloomington, IN 47405, USA e-mail: kaufman@bio.indiana.edu Tel +1-812-8553033, Fax +1-812-8552577, US aut https://doi.org/10.1007/s004270000090 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270000090 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ahzhanov A. Howard Hughes Medical Institute, Department of Biology, Indiana University, Bloomington, IN 47405, USA e-mail: kaufman@bio.indiana.edu Tel +1-812-8553033, Fax +1-812-8552577, US aut NATIONALLICENCE 700 1- Kaufman T. C. Howard Hughes Medical Institute, Department of Biology, Indiana University, Bloomington, IN 47405, USA e-mail: kaufman@bio.indiana.edu Tel +1-812-8553033, Fax +1-812-8552577, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer