caa a22 4500 475768183 CHVBK 20180406123612.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s004270000088 doi (NATIONALLICENCE)springer-10.1007/s004270000088 A cnidarian homologue of translationally controlled tumor protein (P23/TCTP) [Elektronische Daten] [L. Yan, K. Fei, D. Bridge, M.P. Sarras Jr.] Abstract : A protein homologous to P23, or translationally controlled tumor protein (TCTP), was cloned in Hydra vulgaris, the most ancient type of metazoan from which P23/TCTP has been characterized to date. Hydra P23/TCTP is composed of 184 amino acids and is encoded by a single mRNA of 700 bp. This invertebrate P23/TCTP is well conserved compared to those of other invertebrate and vertebrate species. Expression of Hydra P23/TCTP was confirmed by western blot of Hydra cell lysates using a polyclonal antibody against murine recombinant P23/TCTP. Spatial distribution of P23/TCTP mRNA and protein in Hydra was studied using in situ hybridization and immunostaining, respectively. Hydra P23/TCTP expression along the longitudinal body axis is regulated at both the transcriptional and the translational level. High levels of P23/TCTP mRNA were detected in a subpopulation of cells in the body column. In contrast, no mRNA was evident in the differentiated cells of the head and the foot regions. Coincidentally, P23/TCTP protein also concentrates to the body column, with no detectable protein in the head and foot region. However, despite the existence of P23/TCTP mRNA in both the ectoderm and endoderm in the body column, its protein is localized to the endodermal cells, suggesting a regulatory mechanism at the translational level. Taken together, the expression pattern of P23/TCTP in Hydra correlates with regions in which cell proliferation is actively occurring and its expression is excluded from regions where terminal differentiation has occurred. Springer-Verlag Berlin Heidelberg, 2000 Key words P23 nationallicence TCTP nationallicence Cell proliferation nationallicence Hydra nationallicence Differential expression nationallicence Yan L. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kumc.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut Fei K. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kumc.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut Bridge D. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kumc.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut Sarras Jr. M.P. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kumc.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut https://doi.org/10.1007/s004270000088 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270000088 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yan L. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kumc.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut NATIONALLICENCE 700 1- Fei K. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kumc.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut NATIONALLICENCE 700 1- Bridge D. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kumc.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut NATIONALLICENCE 700 1- Sarras Jr M.P. Department of Anatomy and Cell Biology, University of Kansas Medical Center, Kansas City, KS 66160-7400, USA e-mail: msarras@kumc.edu Tel.: +1-913-5882730, Fax: +1-913-5882710, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer