caa a22 4500 475768205 CHVBK 20180406123612.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s004270050307 doi (NATIONALLICENCE)springer-10.1007/s004270050307 An assay system to study migratory behavior of cranial neural crest cells in Xenopus [Elektronische Daten] [A. Borchers, H.-H. Epperlein, D. Wedlich] Abstract : An increasing number of genes are known to show expression in the cranial neural crest area. So far it is very difficult to analyze their effect on neural crest cell migration because of the lack of transplantation techniques. This paper presents a simple method to study the migratory behavior of cranial neural crest cells by homo- and heterotopic transplantations: Green fluorescent protein (GFP) RNA was injected into one blastomere of Xenopus laevis embryos at the 2-cell stage. The cranial neural crest area of stage 14 embryos was transplanted into the head or trunk region of an uninjected host embryo, and the migration was monitored by GFP fluorescence. The transplants were further examined by double immunostaining and confocal microscopy to trace migratory routes inside the embryo, and to exclude contaminations of grafts with foreign tissues. Our results demonstrate that we developed a highly efficient and reproducible technique to study the migratory ability of cranial neural crest cells. It offers the possibility to analyze genes involved in neural crest cell migration by coinjecting their RNA with that of GFP. Springer-Verlag Berlin Heidelberg, 2000 Key words Neural crest nationallicence Transplantation nationallicence Xenopus laevis nationallicence Cell migration nationallicence Borchers A. Department of Biochemistry, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany E-mail: Doris.Wedlich@medizin.uni-ulm.de Tel.: +49-731-5023281, Fax: +49-731-5023277, DE aut Epperlein H.-H Department of Anatomy, University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany, DE aut Wedlich D. Department of Biochemistry, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany E-mail: Doris.Wedlich@medizin.uni-ulm.de Tel.: +49-731-5023281, Fax: +49-731-5023277, DE aut https://doi.org/10.1007/s004270050307 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270050307 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Borchers A. Department of Biochemistry, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany E-mail: Doris.Wedlich@medizin.uni-ulm.de Tel.: +49-731-5023281, Fax: +49-731-5023277, DE aut NATIONALLICENCE 700 1- Epperlein H.-H Department of Anatomy, University of Dresden, Fetscherstrasse 74, 01307 Dresden, Germany, DE aut NATIONALLICENCE 700 1- Wedlich D. Department of Biochemistry, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany E-mail: Doris.Wedlich@medizin.uni-ulm.de Tel.: +49-731-5023281, Fax: +49-731-5023277, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer