caa a22 4500 475768213 CHVBK 20180406123612.0 cr unu---uuuuu 170329e20000301xx s 000 0 eng 10.1007/s004270050302 doi (NATIONALLICENCE)springer-10.1007/s004270050302 Erythropoiesis and unexpected expression pattern of globin genes in the salamander Hynobius retardatus [Elektronische Daten] [M. Yamaguchi, H. Takahashi, M. Wakahara] Abstract : Transition of hemoglobin (Hb) from larval to adult types during the metamorphosis in a salamander Hynobius retardatus has been reported to occur almost independently of thyroid activity, in contrast to the case with many amphibians. In order to obtain further information on the mechanism of the transition in H. retardatus, larval and adult globin cDNAs were cloned, and the globin gene expression was analyzed in normally developing and metamorphosis-arrested animals. Northern hybridization and RT-PCR revealed that larval globin genes were initially expressed 5 days before hatching, and unexpectedly remained expressed even in juveniles 2 years old. The adult globin gene was expressed 19 days after hatching, much earlier than the initiation of morphological metamorphosis. Furthermore, the pattern of globin gene expression in metamorphosis-arrested larvae was almost identical to that in normal controls, suggesting that the transition occurs independently of thyroid hormones. In larvae recovering from anemia, precocious Hb transition, which occurs in Xenopus laevis and Rana catesbeiana, did not occur in H. retardatus. In situ hybridization convincingly demonstrated that the erythropoietic sites are the ventral blood island and the dorsolateral plate at the prehatching stage. During the ontogeny they changed to the liver, kidney, and spleen and were finally restricted to the spleen. Single erythroid cells expressed concurrently larval and adult globin genes, as demonstrated by double in situ hybridization. Thus the transition occurred within a single erythroid cell population, a unique characteristic of H. retardatus. Springer-Verlag Berlin Heidelberg, 2000 Key words Globin genes nationallicence Erythropoiesis nationallicence Metamorphosis nationallicence Thyroid hormone nationallicence Salamander nationallicence Yamaguchi M. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan E-mail: guti@bio.sci.hokudai.ac.jp Tel.: +8-11-7064456, Fax: +8-11-7064455, JP aut Takahashi H. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan, JP aut Wakahara M. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan E-mail: guti@bio.sci.hokudai.ac.jp Tel.: +8-11-7064456, Fax: +8-11-7064455, JP aut https://doi.org/10.1007/s004270050302 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270050302 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi M. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan E-mail: guti@bio.sci.hokudai.ac.jp Tel.: +8-11-7064456, Fax: +8-11-7064455, JP aut NATIONALLICENCE 700 1- Takahashi H. Department of Zoology, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan, JP aut NATIONALLICENCE 700 1- Wakahara M. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan E-mail: guti@bio.sci.hokudai.ac.jp Tel.: +8-11-7064456, Fax: +8-11-7064455, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer