caa a22 4500 475768272 CHVBK 20180406123612.0 cr unu---uuuuu 170329e20000401xx s 000 0 eng 10.1007/s004270050308 doi (NATIONALLICENCE)springer-10.1007/s004270050308 Prox 1 in eye degeneration and sensory organ compensation during development and evolution of the cavefish Astyanax [Elektronische Daten] [W. R. Jeffery, Allen G. Strickler, Stephanie Guiney, Deidre G. Heyser, Stanislav I. Tomarev] Abstract : We have investigated expression of the homeobox gene Prox 1 during eye degeneration and sensory organ compensation in cavefish embryos. The teleost Astyanax mexicanus consists of sighted surface-dwelling forms (surface fish) and several populations of blind cave-dwelling forms (cavefish), which have evolved independently. Eye formation is initiated during cavefish development, but the lens vesicle undergoes apoptosis, and the eye subsequently arrests and degenerates. The requirement of Prox 1 for lens fiber differentiation and γ-crystallin expression in the mouse suggests that changes in the expression of this gene could be involved in cavefish eye degeneration. Surface fish and cavefish embryos stained with a Prox 1 antibody showed Prox 1 expression in the lens, neuroretina, myotomes, heart, hindbrain, and gut, as reported in other vertebrates. We found that Prox 1 expression is not altered during cavefish lens development. Prox 1 protein was detected in the lens vesicle as soon as it formed and persisted until the time of lens degeneration in each cavefish population. The cavefish lens vesicle was also shown to express a γ-crystallin gene, suggesting that Prox 1 is functional in cavefish lens development. In addition to the tissues described above, Prox 1 is expressed in developing taste buds and neuromasts in cavefish, which are enhanced to compensate for blindness. It is concluded that the Prox 1 gene is not involved in lens degeneration, but that expansion of the Prox 1 expression domain occurs during taste bud and neuromast development in cavefish. Springer-Verlag Berlin Heidelberg, 2000 Key words Prox 1 nationallicence Cavefish nationallicence Eye development nationallicence Sensory organ compensation nationallicence Taste buds nationallicence Jeffery W. R. Department of Biology, University of Maryland, College Park, MD 20742, USA E-mail: wj33@umail.umd.edu Tel.: +1-301-4055454, Fax: +1-301-3149358, US aut Strickler Allen G. Department of Biology, University of Maryland, College Park, MD 20742, USA E-mail: wj33@umail.umd.edu Tel.: +1-301-4055454, Fax: +1-301-3149358, US aut Guiney Stephanie Department of Biology, Pennsylvania State University, University Park, PA 16802, USA, US aut Heyser Deidre G. Department of Biology, University of Maryland, College Park, MD 20742, USA E-mail: wj33@umail.umd.edu Tel.: +1-301-4055454, Fax: +1-301-3149358, US aut Tomarev Stanislav I. Laboratory of Molecular and Developmental Biology, National Eye Institute, NIH, Bethesda, MD 20892, USA, US aut https://doi.org/10.1007/s004270050308 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270050308 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jeffery W. R. Department of Biology, University of Maryland, College Park, MD 20742, USA E-mail: wj33@umail.umd.edu Tel.: +1-301-4055454, Fax: +1-301-3149358, US aut NATIONALLICENCE 700 1- Strickler Allen G. Department of Biology, University of Maryland, College Park, MD 20742, USA E-mail: wj33@umail.umd.edu Tel.: +1-301-4055454, Fax: +1-301-3149358, US aut NATIONALLICENCE 700 1- Guiney Stephanie Department of Biology, Pennsylvania State University, University Park, PA 16802, USA, US aut NATIONALLICENCE 700 1- Heyser Deidre G. Department of Biology, University of Maryland, College Park, MD 20742, USA E-mail: wj33@umail.umd.edu Tel.: +1-301-4055454, Fax: +1-301-3149358, US aut NATIONALLICENCE 700 1- Tomarev Stanislav I. Laboratory of Molecular and Developmental Biology, National Eye Institute, NIH, Bethesda, MD 20892, USA, US aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer