caa a22 4500 475768396 CHVBK 20180406123613.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s004270000099 doi (NATIONALLICENCE)springer-10.1007/s004270000099 Graded expression of ceh-14 reporters in the hypodermis is induced by a gonadal signal [Elektronische Daten] [Hiroshi Kagoshima, Ralf Sommer, Brenda J. Reinhart, Giuseppe Cassata, Gary Ruvkun, T. R. Bürglin] Abstract  : ceh-14, a LIM class homeobox gene from Caenorhabditis elegans, is the orthologue of the vertebrate Lhx3/Lhx4 genes. ceh-14 reporter constructs are expressed in several different cell types: head and tail neurons, spermatheca and hypodermis. An intriguing aspect of the hypodermal expression pattern is that it takes the form of a gradient which is strongest in the central body region in L4 to young adult hermaphrodites. Promoter deletion analyses revealed that important regulatory elements for hypodermal expression are located within the transcribed region of ceh-14. Since a large part of the hypodermis is a syncytium, we hypothesized that this expression is triggered in a non-cell-autonomous fashion, a possible source being the underlying gonad. In males, which have a different gonadal organisation, the ceh-14 reporter constructs are expressed in a gradient that is strongest in the tail. By laser ablation of the gonadal precursor cells we found that ceh-14reporter construct expression is eliminated in the hermaphrodite hypodermis, suggesting that the gonad plays a role in the generation of the gradient. Several signaling pathways are known in the gonad and the vulva, thus we crossed the mutations lin-3, egl-17 and lin-12 with the ceh-14 reporter lines. However, the expression of the reporter constructs is not affected in these mutant backgrounds. This suggests that another, presently unknown, signal triggers the graded hypodermal expression. Springer-Verlag Berlin Heidelberg, 2000 Key words LIM homeobox gene nationallicence ceh-14 nationallicence Gradient expression nationallicence Gonadal signal nationallicence Kagoshima Hiroshi Abt. Zellbiologie, Biozentrum der Universität Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland e-mail: Thomas.Buerglin@unibas.ch Tel.: +41-61-2672066 Fax: +41-61-2672078, CH aut Sommer Ralf Abt. für Evolutionsbiologie/IV, Max-Plank-Institut für Entwicklungsbiologie, Spemannstrasse 35/4, 72076 Tübingen, Germany, DE aut Reinhart Brenda J. Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Wellman 8, Fruit Street, Boston, MA 02114, USA, US aut Cassata Giuseppe Abt. Zellbiologie, Biozentrum der Universität Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland e-mail: Thomas.Buerglin@unibas.ch Tel.: +41-61-2672066 Fax: +41-61-2672078, CH aut Ruvkun Gary Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Wellman 8, Fruit Street, Boston, MA 02114, USA, US aut Bürglin T. R. Abt. Zellbiologie, Biozentrum der Universität Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland e-mail: Thomas.Buerglin@unibas.ch Tel.: +41-61-2672066 Fax: +41-61-2672078, CH aut https://doi.org/10.1007/s004270000099 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s004270000099 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kagoshima Hiroshi Abt. Zellbiologie, Biozentrum der Universität Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland e-mail: Thomas.Buerglin@unibas.ch Tel.: +41-61-2672066 Fax: +41-61-2672078, CH aut NATIONALLICENCE 700 1- Sommer Ralf Abt. für Evolutionsbiologie/IV, Max-Plank-Institut für Entwicklungsbiologie, Spemannstrasse 35/4, 72076 Tübingen, Germany, DE aut NATIONALLICENCE 700 1- Reinhart Brenda J. Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Wellman 8, Fruit Street, Boston, MA 02114, USA, US aut NATIONALLICENCE 700 1- Cassata Giuseppe Abt. Zellbiologie, Biozentrum der Universität Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland e-mail: Thomas.Buerglin@unibas.ch Tel.: +41-61-2672066 Fax: +41-61-2672078, CH aut NATIONALLICENCE 700 1- Ruvkun Gary Department of Molecular Biology, Massachusetts General Hospital and Department of Genetics, Harvard Medical School, Wellman 8, Fruit Street, Boston, MA 02114, USA, US aut NATIONALLICENCE 700 1- Bürglin T. R. Abt. Zellbiologie, Biozentrum der Universität Basel, Klingelbergstrasse 70, 4056 Basel, Switzerland e-mail: Thomas.Buerglin@unibas.ch Tel.: +41-61-2672066 Fax: +41-61-2672078, CH aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer