caa a22 4500 475771338 CHVBK 20180406123618.0 cr unu---uuuuu 170329e20000901xx s 000 0 eng 10.1007/s001250051508 doi (NATIONALLICENCE)springer-10.1007/s001250051508 Expression of peroxisome proliferator-activated receptor γ (PPARγ) in normal human pancreatic islet cells [Elektronische Daten] [M. Dubois, F. Pattou, J. Kerr-Conte, V. Gmyr, B. Vandewalle, P. Desreumaux, J. Auwerx, K. Schoonjans, J. Lefebvre] Abstract : Aims/hypothesis. Thiazolidinediones are reported to improve pancreatic islet morphology and beta-cell function in rodents, supporting the hypothesis of a direct action of thiazolidinediones on endocrine islet cells. In this study we examined the expression of the peroxisome proliferator-activated receptor γ, a nuclear receptor that is activated by naturally occurring fatty acids and synthetic thiazolidinediones, in normal human endocrine pancreatic cells. Methods. Human islets were isolated from pancreata harvested in ten brain-dead lean non-diabetic adult donors. We analysed the gene and protein expression of the human peroxisome proliferator-activated receptor γ and evaluated the effects of peroxisome proliferator-activated receptor γ agonist on insulin secretion in human islet preparations. Results. The RT-PCR carried out on total RNA from four distinct human islet preparations demonstrated the presence of peroxisome proliferator-activated receptor γ mRNA. Western blot analysis showed the consistent expression of peroxisome proliferator-activated receptor γ protein. Peroxisome proliferator-activated receptor γ was shown to be present in all three endocrine cell types studied (alpha, beta and delta cells) by immunohistochemistry. Conclusion/interpretation. We found that peroxisome proliferator-activated receptor γ is highly expressed in human islet endocrine cells, both at the mRNA and protein levels. These results support the hypothesis of a direct influence of peroxisome proliferator-activated receptor γ agonist on human pancreatic endocrine cells. [Diabetologia (2000) 43: 1165-1169] Springer-Verlag Berlin Heidelberg, 2000 Keywords Type II diabetes nationallicence peroxisome proliferator-activated receptor γ nationallicence human pancreas nationallicence islets nationallicence beta cell nationallicence thiazolidinediones nationallicence troglitazone nationallicence rosiglitazone nationallicence Dubois M. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut Pattou F. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut Kerr-Conte J. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut Gmyr V. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut Vandewalle B. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut Desreumaux P. Research Laboratory of Inflammatory Intestinal Disorders (CRI4U004B), University Hospital Centre of Lille, Lille, France, FR aut Auwerx J. Institut of Genetics and Molecular and Cellular Biology (IGBMC), CNRS, INSERM, ULP, Illkirch, France, FR aut Schoonjans K. Institut of Genetics and Molecular and Cellular Biology (IGBMC), CNRS, INSERM, ULP, Illkirch, France, FR aut Lefebvre J. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut https://doi.org/10.1007/s001250051508 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051508 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dubois M. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut NATIONALLICENCE 700 1- Pattou F. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut NATIONALLICENCE 700 1- Kerr-Conte J. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut NATIONALLICENCE 700 1- Gmyr V. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut NATIONALLICENCE 700 1- Vandewalle B. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut NATIONALLICENCE 700 1- Desreumaux P. Research Laboratory of Inflammatory Intestinal Disorders (CRI4U004B), University Hospital Centre of Lille, Lille, France, FR aut NATIONALLICENCE 700 1- Auwerx J. Institut of Genetics and Molecular and Cellular Biology (IGBMC), CNRS, INSERM, ULP, Illkirch, France, FR aut NATIONALLICENCE 700 1- Schoonjans K. Institut of Genetics and Molecular and Cellular Biology (IGBMC), CNRS, INSERM, ULP, Illkirch, France, FR aut NATIONALLICENCE 700 1- Lefebvre J. UPRES 1048, University Hospital Centre of Lille, Lille, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer