caa a22 4500 475771478 CHVBK 20180406123618.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001250051464 doi (NATIONALLICENCE)springer-10.1007/s001250051464 The lipoprotein lipase activator, NO-1886, suppresses fat accumulation and insulin resistance in rats fed a high-fat diet [Elektronische Daten] [M. Kusunoki, T. Hara, K Tsutsumi, T. Nakamura, T Miyata, F. Sakakibara, S. Sakamoto, H. Ogawa, Y. Nakaya, L. H. Storlien] Abstract : Aims/hypothesis. Fat balance is critical in the aetiology of obesity and related diseases. Lipoprotein lipase is of major importance in lipid metabolism. The aim of this study was to investigate the long-term effects of the lipoprotein lipase activator, NO-1886, on substrate utilisation, adiposity and insulin action in rats fed a high-fat diet.¶Methods. Male, Sprague-Dawley rats were fed for 10 weeks on a chow diet or a high-fat diet with, or without, NO-1886 (50 mg · kg-1· day-1). Weight gain, fat accumulation and both hormone-sensitive and lipoprotein, lipase activities were measured. Insulin action was assessed by the euglycaemic hyperinsulinaemic clamp and metabolic rate/substrate utilisation by open-circuit respirometry.¶Results. Compared with chow-fed controls, a high-fat diet increased weight gain, an effect lessened by NO-1886 [weight gain (g): chow, 37 ± 3, high-fat, 222 ± 9; high-fat + NO-1886, 109 ± 6, all groups differed p < 0.001]. A similar pattern existed for fat accumulation [visceral fat (g): chow, 35.9 ± 3.2; high-fat, 81.9 ± 6.6; high-fat + NO-1886, 52.3 ± 4.7, p < 0.01 high-fat vs the other groups]. A high-fat diet induced whole-body insulin resistance (clamp glucose infusion rate: 4.8 ± 1.3 mg · kg-1· min-1 vs 10.6 ± 1.1 for the chow group, p < 0.01) with NO-1886 lessening this effect (8.3 ± 0.5, p < 0.05 vs high-fat). The 24-h respiratory quotient was lower in the high-fat + NO-1886 group (0.825 ± 0.010) compared with high-fat alone (0.849 ± 0.004, p < 0.05). A high-fat diet increased lipoprotein and hormone-sensitive, lipase activities in epididymal fat, an effect not altered by NO-1886. In myocardium and skeletal muscle a high-fat diet lowered lipoprotein lipase activity, an effect lessened by NO-1886.¶Conclusion/interpretation: Lipoprotein lipase activators could have potential benefits for the treatment of obesity by increasing fat utilisation. [Diabetologia (2000) 43: 875-880] Springer-Verlag Berlin Heidelberg, 2000 Keywords Hormone sensitive lipase, obesity, respiratory quotient, skeletal muscle, visceral fat nationallicence Kusunoki M. The First Department of Internal Medicine, Aichi Medical University, Aichi, Japan, JP aut Hara T. The First Department of Internal Medicine, Aichi Medical University, Aichi, Japan, JP aut Tsutsumi K. The Nutrition Research Institute, Otsuka Pharmaceutical Factory, Tokushima, Japan, JP aut Nakamura T. Laboratory of Biomedical Control, Research Institute for Electronic Science, Hokkaido University, Sapporo, Japan, JP aut Miyata T. Division of Vascular Surgery, Department of Surgery, Faculty of Medicine, The University of Tokyo, Tokyo, Japan, JP aut Sakakibara F. The First Department of Internal Medicine, Aichi Medical University, Aichi, Japan, JP aut Sakamoto S. Department of Nutrition, School of Medicine, Tokushima University, Tokushima, Japan, JP aut Ogawa H. The First Department of Internal Medicine, Aichi Medical University, Aichi, Japan, JP aut Nakaya Y. Department of Nutrition, School of Medicine, Tokushima University, Tokushima, Japan, JP aut Storlien L. H. Metabolic Research Centre, Dept. Biomedical Science, University of Wollongong, Australia, AU aut https://doi.org/10.1007/s001250051464 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051464 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kusunoki M. The First Department of Internal Medicine, Aichi Medical University, Aichi, Japan, JP aut NATIONALLICENCE 700 1- Hara T. The First Department of Internal Medicine, Aichi Medical University, Aichi, Japan, JP aut NATIONALLICENCE 700 1- Tsutsumi K. The Nutrition Research Institute, Otsuka Pharmaceutical Factory, Tokushima, Japan, JP aut NATIONALLICENCE 700 1- Nakamura T. Laboratory of Biomedical Control, Research Institute for Electronic Science, Hokkaido University, Sapporo, Japan, JP aut NATIONALLICENCE 700 1- Miyata T. Division of Vascular Surgery, Department of Surgery, Faculty of Medicine, The University of Tokyo, Tokyo, Japan, JP aut NATIONALLICENCE 700 1- Sakakibara F. The First Department of Internal Medicine, Aichi Medical University, Aichi, Japan, JP aut NATIONALLICENCE 700 1- Sakamoto S. Department of Nutrition, School of Medicine, Tokushima University, Tokushima, Japan, JP aut NATIONALLICENCE 700 1- Ogawa H. The First Department of Internal Medicine, Aichi Medical University, Aichi, Japan, JP aut NATIONALLICENCE 700 1- Nakaya Y. Department of Nutrition, School of Medicine, Tokushima University, Tokushima, Japan, JP aut NATIONALLICENCE 700 1- Storlien L. H. Metabolic Research Centre, Dept. Biomedical Science, University of Wollongong, Australia, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer