caa a22 4500 475771494 CHVBK 20180406123618.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001250051472 doi (NATIONALLICENCE)springer-10.1007/s001250051472 Age-dependent changes in phenotypes and candidate gene analysis in a polygenic animal model of Type II diabetes mellitus; NSY mouse [Elektronische Daten] [H. Ueda, H. Ikegami, Y. Kawaguchi, T. Fujisawa, K. Nojima, N. Babaya, K. Yamada, M. Shibata, E. Yamato, T. Ogihara] Aims/hypothesis. The Nagoya-Shibata-Yasuda (NSY) mouse closely mimics human Type II (non-insulin-dependent) diabetes mellitus in that the onset is age-dependent, the animals are not severely obese, and both insulin resistance and impaired insulin response to glucose contribute to disease development. The aim of this study was to clarify the influence of age on the pathogenesis of diabetes and to analyse a candidate gene for Type II diabetes in this strain.¶Methods. Several phenotypic characteristics related to diabetes mellitus were monitored longitudinally in male NSY and control C3H/He mice. The nucleotide sequence of Glut4, a candidate gene for Nidd1nsy (a susceptibility gene for Type II diabetes) on Chromosome 11, encoding insulin-sensitive glucose transporter, was determined in NSY and C3H mice.¶Results. Glucose intolerance worsened with age, and fasting blood glucose and fasting plasma insulin concentration increased with age in NSY mice. Pancreatic insulin content increased until 24 weeks of age but then decreased at 48 weeks of age in NSY mice. The hypoglycaemic response to insulin was statistically significantly smaller in NSY than in C3H/He mice. The nucleotide sequence of GLUT4 cDNA was identical in NSY and C3H/He mice, but both were different from the sequence reported previously.¶Conclusion/interpretation. Insulin secretion and insulin resistance, as well as ageing possibly play an important part in the disease development in NSY mice. A decline of pancreatic insulin content in older age might cause the relative insulin deficiency in this strain. Nucleotide sequencing suggests that Glut4 is unlikely to be a candidate gene for Nidd1nsy. [Diabetologia (2000) 43: 932-938] Springer-Verlag Berlin Heidelberg, 2000 Keywords NSY mouse, ageing, animal model, insulin resistance, glucose transporter 4 nationallicence Ueda H. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut Ikegami H. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut Kawaguchi Y. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut Fujisawa T. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut Nojima K. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut Babaya N. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut Yamada K. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut Shibata M. Department of Health, Aichi-gakuin University, College of General Education, Aichi, Japan, JP aut Yamato E. Department of Nutrition and Physiological Chemistry, Osaka University Medical School, Osaka, Japan, JP aut Ogihara T. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut https://doi.org/10.1007/s001250051472 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051472 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ueda H. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut NATIONALLICENCE 700 1- Ikegami H. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut NATIONALLICENCE 700 1- Kawaguchi Y. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut NATIONALLICENCE 700 1- Fujisawa T. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut NATIONALLICENCE 700 1- Nojima K. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut NATIONALLICENCE 700 1- Babaya N. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut NATIONALLICENCE 700 1- Yamada K. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut NATIONALLICENCE 700 1- Shibata M. Department of Health, Aichi-gakuin University, College of General Education, Aichi, Japan, JP aut NATIONALLICENCE 700 1- Yamato E. Department of Nutrition and Physiological Chemistry, Osaka University Medical School, Osaka, Japan, JP aut NATIONALLICENCE 700 1- Ogihara T. Department of Geriatric Medicine, Osaka University Medical School, Osaka, Japan, JP aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer