caa a22 4500 475771621 CHVBK 20180406123619.0 cr unu---uuuuu 170329e20000701xx s 000 0 eng 10.1007/s001250051462 doi (NATIONALLICENCE)springer-10.1007/s001250051462 Interaction between adrenal glucocorticoids and parasympathetic activation in mediating hyperinsulinaemia during long-term central neuropeptide Y infusion in rats [Elektronische Daten] [A. Sainsbury, D. Wilks, G. J. Cooney] Aims/hypothesis. Hypothalamic neuropeptide Y is implicated in the aetiology of obesity and insulin resistance because of its hyperinsulinaemic, hyperphagic effects. We investigated the interaction of adrenal glucocorticoids and the parasympathetic nervous system in the hyperinsulinaemia caused by neuropeptide Y infusion in rats.¶Methods. Neuropeptide Y was intracerebroventricularly given to normal or adrenalectomised rats for 3-6 days with pair-feeding, with or without subcutaneous dexamethasone infusion. We measured basal and intravenous glucose-induced insulinaemia and the effect of prior atropine injection.¶Results. Neuropeptide Y increased basal plasma insulin and C-peptide concentrations (380 ± 90 and 1000 ± 60 pmol/l, vs 190 ± 20 and 590 ± 50 pmol/l in controls, p < 0.05). Neuropeptide Y also increased the plasma concentrations of these hormones as early as 60 s after glucose injection (1630 ± 170 and 3200 ± 170 pmol/l for insulin and C peptide, respectively, vs 1080 ± 80 and 1860 ± 130 pmol/l in controls, p < 0.05). Atropine reversed the effect of neuropeptide Y on basal plasma insulin and C-peptide concentrations but had no effect on post-glucose plasma concentrations. The hyperinsulinaemic effects of neuropeptide Y were prevented by adrenalectomy, but were restored by dexamethasone infusion. Dexamethasone in itself did not statistically significantly increase insulinaemia in adrenalectomised rats. As in intact rats, atropine attenuated the basal hyperinsulinaemia of adrenalectomised rats that had been infused with neuropeptide Y and dexamethasone but had no effect on post-glucose hyperinsulinaemia.¶Conclusion/interpretation. These data suggest firstly that neuropeptide Y infused centrally induces basal hyperinsulinaemia in rats through glucocorticoid-dependant parasympathetic activation to the pancreas. Secondly, neuropeptide Y potentiates glucose-induced insulinaemia through a pathway dependant on adrenal glucocorticoids that cannot be reversed by short-term blockade of the increased parasympathetic tonus. [Diabetologia (2000) 43: 859-865] Springer-Verlag Berlin Heidelberg, 2000 Keywords Neuropeptide Y, intracerebroventricular, hyperinsulinaemia, adrenalectomy, atropine, dexamethasone, rat nationallicence Sainsbury A. Diabetes Research Group, Garvan Institute of Medical Research, Darlinghurst, Sydney, Australia, AU aut Wilks D. Diabetes Research Group, Garvan Institute of Medical Research, Darlinghurst, Sydney, Australia, AU aut Cooney G. J. Diabetes Research Group, Garvan Institute of Medical Research, Darlinghurst, Sydney, Australia, AU aut https://doi.org/10.1007/s001250051462 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051462 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sainsbury A. Diabetes Research Group, Garvan Institute of Medical Research, Darlinghurst, Sydney, Australia, AU aut NATIONALLICENCE 700 1- Wilks D. Diabetes Research Group, Garvan Institute of Medical Research, Darlinghurst, Sydney, Australia, AU aut NATIONALLICENCE 700 1- Cooney G. J. Diabetes Research Group, Garvan Institute of Medical Research, Darlinghurst, Sydney, Australia, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer