caa a22 4500 475772091 CHVBK 20180406123620.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001250051528 doi (NATIONALLICENCE)springer-10.1007/s001250051528 Oscillations in oxygen tension and insulin release of individual pancreatic ob/ob mouse islets [Elektronische Daten] [H. Ortsäter, P. Liss, P. E. Lund, K. E. O. Åkerman, P. Bergsten] Aims/hypothesis. The role of beta-cell metabolism for generation of oscillatory insulin release was investigated by simultaneous measurements of oxygen tension (pO2) and insulin release from individual islets of Langerhans.¶Methods. Individual islets isolated from the ob/ob-mice were perifused. Insulin in the perifusate was measured with a sensitive ELISA and pO2 with a modified Clark-type electrode inserted into the islets.¶Results. In the presence of 3 mmol/l d-glucose, pO2 was 102 ± 9 mmHg and oscillatory (0.26 ± 0.04 oscillations/min). Corresponding insulin measurements showed oscillatory release with similar periodicity (0.25 ± 0.02 oscillations/min). When the d-glucose concentration was increased to 11 mmol/l, pO2 decreased by 30 % to 72 ± 10 mmHg with maintained frequency of the oscillations. Corresponding insulin secretory rate rose from 5 ± 2 to 131 ± 16 pmol · g-1· s-1 leaving the frequency of the insulin pulses unaffected. The magnitude of glucose-induced change in pO2 varied between islets but was positively correlated to the amount of insulin released (r 2 = 0.85). When 1 mmol/l tolbutamide was added to the perifusion medium containing 11 mmol/l glucose no change in average oscillatory pO2 was observed despite a doubling in the secretory rate. When 8 mmol/l 3-oxymethyl glucose was added to perifusion medium containing 3 mmol/l d-glucose, neither pO2 nor insulin release of the islets were changed. Temporal analysis of oscillations in pO2 and insulin release revealed that maximum respiration correlated to maximum or close to maximum insulin release.¶Conclusion/interpretation. The temporal relation between oscillations in pO2 and insulin release supports a role for metabolic oscillations in the generation of pulsatile insulin release. [Diabetologia (2000) 43: 1313-1318] Springer-Verlag Berlin Heidelberg, 2000 Keywords Oscillation, metabolism, oxygen tension, insulin release, islet, glucose, tolbutamide, heterogeneity, Clark electrode, ELISA nationallicence Ortsäter H. Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden, SE aut Liss P. Department of Physiology, Uppsala University, Uppsala, Sweden, SE aut Lund P. E. Department of Physiology, Uppsala University, Uppsala, Sweden, SE aut Åkerman K. E. O. Department of Physiology, Uppsala University, Uppsala, Sweden, SE aut Bergsten P. Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden, SE aut https://doi.org/10.1007/s001250051528 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051528 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ortsäter H. Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden, SE aut NATIONALLICENCE 700 1- Liss P. Department of Physiology, Uppsala University, Uppsala, Sweden, SE aut NATIONALLICENCE 700 1- Lund P. E. Department of Physiology, Uppsala University, Uppsala, Sweden, SE aut NATIONALLICENCE 700 1- Åkerman K. E. O. Department of Physiology, Uppsala University, Uppsala, Sweden, SE aut NATIONALLICENCE 700 1- Bergsten P. Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden, SE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer