caa a22 4500 475772113 CHVBK 20180406123620.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001250051521 doi (NATIONALLICENCE)springer-10.1007/s001250051521 Effect of long-term dietary protein intake on glucose metabolism in humans [Elektronische Daten] [T. Linn, B. Santosa, D. Grönemeyer, S. Aygen, N. Scholz, M. Busch, R. G. Bretzel] Aims/hypothesis. A meal rich in protein stimulates insulin secretion. Long-term effects of dietary protein on insulin release and glucose metabolism are, however, still not known. Our study focussed on the effect of different protein intake on pancreatic insulin secretion capacity, glycogen turnover and gluconeogenesis.¶Methods. Subjects with constant (6 months) dietary protein of 1.87 ± 0.26 g · kg-1· day-1 (1.25-2.41) named high protein group and with 0.74 ± 0.08 (0.57-0.80), normal protein group, were identified by a food questionnaire and were matched (n = 9) according to sex, age and calorie intake. They underwent an intravenous glucose tolerance test and a euglycaemic hyperinsulinaemic clamp with infusion of [6,6-2H2]-glucose combined with indirect calorimetry. To estimate net gluconeogenesis the usual diet was enriched by deuterated water or U-[13C6]-glucose and breath and plasma were sampled.¶Results. Glucose-stimulated insulin secretion was increased in the high protein group (516 ± 45 pmol/l vs 305 ± 32, p = 0.012) due to reduced glucose threshold of the endocrine beta cells (4.2 ± 0.5 mmol/l vs 4.9 ± 0.3, p = 0.031). Endogeneous glucose output was increased by 12 % (p = 0.009) at 40 pmol/l plasma insulin in the high protein group, but not at higher insulin concentration whereas overall glucose disposal was reduced. Fasting plasma glucagon was 34 % increased in the high protein group (p = 0.038). Fractional gluconeogenesis was increased by 40 % in subjects receiving a high protein diet as determined by both methods.¶Conclusion/interpretation. High protein diet is accompanied by increased stimulation of glucagon and insulin within the endocrine pancreas, high glycogen turnover and stimulation of gluconeogenesis. [Diabetologia (2000) 43: 1257-1265] Springer-Verlag Berlin Heidelberg, 2000 Keywords Endogeneous insulin, hepatic glucose production, high protein diet, normal protein diet nationallicence Linn T. Clinical Research Unit, Medical Clinic 3, Giessen, Germany, DE aut Santosa B. Clinical Research Unit, Medical Clinic 3, Giessen, Germany, DE aut Grönemeyer D. Department of Radiology and MicroTherapy. University Witten/Herdecke, Germany, DE aut Aygen S. Institute of Clinical Chemistry, University Witten/Herdecke, Germany, DE aut Scholz N. Division of Diabetes and Metabolism, Medical Clinic 3, Giessen, Germany, DE aut Busch M. Department of Radiology and MicroTherapy. University Witten/Herdecke, Germany, DE aut Bretzel R. G. Division of Diabetes and Metabolism, Medical Clinic 3, Giessen, Germany, DE aut https://doi.org/10.1007/s001250051521 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051521 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Linn T. Clinical Research Unit, Medical Clinic 3, Giessen, Germany, DE aut NATIONALLICENCE 700 1- Santosa B. Clinical Research Unit, Medical Clinic 3, Giessen, Germany, DE aut NATIONALLICENCE 700 1- Grönemeyer D. Department of Radiology and MicroTherapy. University Witten/Herdecke, Germany, DE aut NATIONALLICENCE 700 1- Aygen S. Institute of Clinical Chemistry, University Witten/Herdecke, Germany, DE aut NATIONALLICENCE 700 1- Scholz N. Division of Diabetes and Metabolism, Medical Clinic 3, Giessen, Germany, DE aut NATIONALLICENCE 700 1- Busch M. Department of Radiology and MicroTherapy. University Witten/Herdecke, Germany, DE aut NATIONALLICENCE 700 1- Bretzel R. G. Division of Diabetes and Metabolism, Medical Clinic 3, Giessen, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer