caa a22 4500 475772628 CHVBK 20180406123621.0 cr unu---uuuuu 170329e20000801xx s 000 0 eng 10.1007/s001250051481 doi (NATIONALLICENCE)springer-10.1007/s001250051481 Association between microsomal triglyceride transfer protein gene polymorphism and the biological features of liver steatosis in patients with Type II diabetes [Elektronische Daten] [S. Bernard, S. Touzet, I. Personne, V. Lapras, P. J. Bondon, F. Berthezène, P. Moulin] Aims/hypothesis. Non-alcoholic steatohepatitis is frequent in Type II (non-insulin-dependent) diabetes mellitus and can lead to fibrosis and cirrhosis. The interindividual variability in the occurrence of non-alcoholic steatohepatitis suggests, however, a genetic modulation. Microsomal triglyceride transfer protein (MTP) is necessary for the assembly and secretion of VLDL and when the protein is not functional, such as in abetalipoproteinaemia, a steatohepatitis occurs. We therefore assessed the association between a functional polymorphism in the promoter region of MTP gene (-493 G/T) and the biological features of steatohepatitis in Type II diabetic patients. Methods. We studied 271 patients with Type II diabetes. Determination of -493 G/T polymorphism was made by PCR-RFLP. Increased liver enzymes were used as surrogates of liver steatosis and alanine aminotransferase concentration was the outcome variable for the multivariate analysis. Liver ultrasonography was available for a subgroup of patients with newly diagnosed diabetes. Results. The proportion of patients with increased alanine aminotransferase was higher in GG than in GT and TT subgroups (23 %, 11 % and 6 %, respectively, p = 0.01). Additionally, patients with high alanine aminotransferase concentrations were more likely to be young (p = 0.01), male (p = 0.001), obese (p = 0.04) and have low HDL-cholesterol (p = 0.01). In multivariate analysis, the MTP genotype was independently associated with alanine aminotransferase concentration (p = 0.0023) as well as sex and body mass index but not HDL-cholesterol. Conclusion/interpretation. The -493 G/T MTP gene polymorphism is associated with biological surrogates of steatohepatitis in patients with Type II diabetes. The G allele which is responsible for a decrease in MTP gene transcription is prone to increase the intrahepatic triglycerides content, conferring by this a genetic susceptibility for steatohepatitis. [Diabetologia (2000) 43: 995-999] Springer-Verlag Berlin Heidelberg, 2000 Keywords Microsomal triglyceride transfer protein nationallicence Gene polymorphism nationallicence Genetic susceptibility nationallicence Non-alcoholic steatohepatitis nationallicence Type II diabetes mellitus nationallicence Bernard S. Department of Endocrinology and Nutrition, Cardiovascular Hospital Louis-Pradel, Bron, France, FR aut Touzet S. Department of Medical Information, Lyons, France, FR aut Personne I. Department of Endocrinology and Nutrition, Cardiovascular Hospital Louis-Pradel, Bron, France, FR aut Lapras V. Department of Radiology, Antiquaille Hospital, Lyons, France, FR aut Bondon P. J. Biochemistry Laboratory, Antiquaille Hospital, Lyons, France, FR aut Berthezène F. Department of Endocrinology and Nutrition, Cardiovascular Hospital Louis-Pradel, Bron, France, FR aut Moulin P. Department of Endocrinology and Nutrition, Cardiovascular Hospital Louis-Pradel, Bron, France, FR aut https://doi.org/10.1007/s001250051481 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051481 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bernard S. Department of Endocrinology and Nutrition, Cardiovascular Hospital Louis-Pradel, Bron, France, FR aut NATIONALLICENCE 700 1- Touzet S. Department of Medical Information, Lyons, France, FR aut NATIONALLICENCE 700 1- Personne I. Department of Endocrinology and Nutrition, Cardiovascular Hospital Louis-Pradel, Bron, France, FR aut NATIONALLICENCE 700 1- Lapras V. Department of Radiology, Antiquaille Hospital, Lyons, France, FR aut NATIONALLICENCE 700 1- Bondon P. J. Biochemistry Laboratory, Antiquaille Hospital, Lyons, France, FR aut NATIONALLICENCE 700 1- Berthezène F. Department of Endocrinology and Nutrition, Cardiovascular Hospital Louis-Pradel, Bron, France, FR aut NATIONALLICENCE 700 1- Moulin P. Department of Endocrinology and Nutrition, Cardiovascular Hospital Louis-Pradel, Bron, France, FR aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer