caa a22 4500 475772768 CHVBK 20180406123622.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s001250051347 doi (NATIONALLICENCE)springer-10.1007/s001250051347 Short-term treatment with GLP-1 increases pulsatile insulin secretion in Type II diabetes with no effect on orderliness [Elektronische Daten] [C. B. Juhl, O. Schmitz, S. Pincus, J. J. Holst, J. Veldhuis, N. Pørksen] Aims/hypothesis. The enteric incretin hormone, glucagon-like peptide-1 (GLP-1), is a potent insulin secretagogue in healthy humans and patients with Type II (non-insulin-dependent) diabetes mellitus. In this study we assessed the impact of short-term GLP-1 infusion on pulsatile insulin secretion in Type II diabetic patients. Methods. Type II diabetic patients (n = 8) were studied in a randomised cross-over design. Plasma insulin concentration time series were obtained during basal conditions and during infusion with saline or GLP-1 (1.2 pmol/l · kg-1· min-1) on 2 separate days. Plasma glucose was clamped at the initial concentration by a variable glucose infusion. Serum insulin concentration time series were evaluated by deconvolution analysis, autocorrelation analysis, spectral analysis and approximate entropy. Results. Serum insulin concentrations increased by approximately 100 % during GLP-1 infusion. Pulsatile insulin secretion was increased as measured by secretory burst mass (19.3 ± 3.8 vs 53.0 ± 10.7 pmol/l/pulse, p = 0.02) and secretory burst amplitude (7.7 ± 1.5 vs 21.1 ± 4.3 pmol/l/min, p = 0.02). A similar increase in basal insulin secretion was observed (3.6 ± 0.9 vs 10.2 ± 2.2 pmol/l/min, p = 0.004) with no changes in the fraction of insulin delivered in pulses (0.50 ± 0.06 vs 0.49 ± 0.02, p = 0.84). Regularity of secretion was unchanged as measured by spectral analysis (normalised spectral power: 5.9 ± 0.6 vs 6.3 ± 0.8, p = 0.86), autocorrelation analysis (autocorrelation coefficient: 0.19 ± 0.04 vs 0.18 ± 0.05, p = 0.66) and the approximate entropy statistic (1.48 ± 0.02 vs 1.51 ± 0.02, p = 0.86). Conclusion/interpretation. Short-term stimulation with GLP-1 jointly increases pulsatile and basal insulin secretion, maintaining but not improving system regularity in Type II diabetic patients. [Diabetologia (2000) 43: 583-588] Springer-Verlag Berlin Heidelberg, 2000 Keywords GLP-1 nationallicence Insulin nationallicence pulsatility nationallicence insulin secretion nationallicence time series nationallicence Type II diabetes nationallicence human nationallicence Juhl C. B. Department of Medicine M (Endocrinology and Diabetes), University Hospital of Aarhus, Aarhus, Denmark, DK aut Schmitz O. Department of Medicine M (Endocrinology and Diabetes), University Hospital of Aarhus, Aarhus, Denmark, DK aut Pincus S. Guilford, Connecticut, USA, US aut Holst J. J. Department of Medical Physiology, University of Copenhagen, Denmark, DK aut Veldhuis J. Endocrinology Division, General Clinical Research Center, University of Virginia, Charlottesville, Virginia, USA, US aut Pørksen N. Department of Medicine M (Endocrinology and Diabetes), University Hospital of Aarhus, Aarhus, Denmark, DK aut https://doi.org/10.1007/s001250051347 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051347 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Juhl C. B. Department of Medicine M (Endocrinology and Diabetes), University Hospital of Aarhus, Aarhus, Denmark, DK aut NATIONALLICENCE 700 1- Schmitz O. Department of Medicine M (Endocrinology and Diabetes), University Hospital of Aarhus, Aarhus, Denmark, DK aut NATIONALLICENCE 700 1- Pincus S. Guilford, Connecticut, USA, US aut NATIONALLICENCE 700 1- Holst J. J. Department of Medical Physiology, University of Copenhagen, Denmark, DK aut NATIONALLICENCE 700 1- Veldhuis J. Endocrinology Division, General Clinical Research Center, University of Virginia, Charlottesville, Virginia, USA, US aut NATIONALLICENCE 700 1- Pørksen N. Department of Medicine M (Endocrinology and Diabetes), University Hospital of Aarhus, Aarhus, Denmark, DK aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer