caa a22 4500 475772776 CHVBK 20180406123622.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s001250051346 doi (NATIONALLICENCE)springer-10.1007/s001250051346 Effects of gestational diabetes on human placental glucose uptake, transfer, and utilisation [Elektronische Daten] [D. T. D. Osmond, C. J. Nolan, R. G. King, S. P. Brennecke, N. M. Gude] Aims/hypothesis. Gestational diabetes is associated with complications for the offspring before, during and after delivery. Poor maternal glucose control, however, is a weak predictor of these complications. Given its position at the interface of the maternal and fetal circulations, the placenta possibly plays a crucial part in protecting the fetus from adverse effects from the maternal diabetic milieu. We hypothesised that gestational diabetes may result in changes in placental function, particularly with respect to the uptake, transfer, and/or utilisation of glucose. We aimed to examine glucose transport and utilisation in intact human placental lobules from women with gestational diabetes and those from normal pregnancies. Method. Dual perfusion of an isolated placental lobule was done on placentae from diet treated gestational diabetic (n = 7) and normal pregnant patients (n = 9) using maternal glucose concentrations of 4, 8, 16 and 24 mmol/l in random order over a 4-h experiment. Results were expressed in μmol · min-1· g-1. Results. d-glucose uptake from the maternal circulation (control 0.492 vs gestational diabetes mellitus 0.248, at 8 mmol/l maternal glucose), d-glucose utilisation by the placenta (0.255 vs 0.129), d-glucose transfer to the fetal circulation (direct 0.979 vs 0.402; net transfer 0.269 vs 0.118) and l-lactate maternal release into both the fetal (0.052 vs 0.042) and maternal (0.255 vs 0.129) circulation were significantly reduced during in vitro perfusion of placentae from patients with gestational diabetic pregnancies. Transfer of 3H-l-glucose also significantly reduced in the diabetic group (8.1 % vs 2.6 %). Conclusion/interpretation. These results suggest that placental transport and metabolism of d-glucose is altered during gestational diabetes. [Diabetologia (2000) 43: 576-582] Springer-Verlag Berlin Heidelberg, 2000 Keywords Gestational diabetes nationallicence human placenta nationallicence glucose metabolism nationallicence in vitro perfusion nationallicence Osmond D. T. D. Department of Perinatal Medicine, Royal Women's Hospital, Carlton Victoria, Australia, AU aut Nolan C. J. Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women's Hospital, Carlton Victoria, Australia, AU aut King R. G. Department of Pharmacology, Monash University, Clayton Victoria, Australia, AU aut Brennecke S. P. Consultant Endocrinologist, Geelong Hospital, Geelong Victoria, Australia, AU aut Gude N. M. Department of Perinatal Medicine, Royal Women's Hospital, Carlton Victoria, Australia, AU aut https://doi.org/10.1007/s001250051346 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051346 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Osmond D. T. D. Department of Perinatal Medicine, Royal Women's Hospital, Carlton Victoria, Australia, AU aut NATIONALLICENCE 700 1- Nolan C. J. Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women's Hospital, Carlton Victoria, Australia, AU aut NATIONALLICENCE 700 1- King R. G. Department of Pharmacology, Monash University, Clayton Victoria, Australia, AU aut NATIONALLICENCE 700 1- Brennecke S. P. Consultant Endocrinologist, Geelong Hospital, Geelong Victoria, Australia, AU aut NATIONALLICENCE 700 1- Gude N. M. Department of Perinatal Medicine, Royal Women's Hospital, Carlton Victoria, Australia, AU aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer