caa a22 4500 475772857 CHVBK 20180406123622.0 cr unu---uuuuu 170329e20000501xx s 000 0 eng 10.1007/s001250051354 doi (NATIONALLICENCE)springer-10.1007/s001250051354 Neuronal and endothelial nitric oxide synthase expression in outer medulla of streptozotocin-induced diabetic rat kidney [Elektronische Daten] [S. J. Shin, F. J. Lai, J. D. Wen, P. J. Hsiao, M. C. Hsieh, T. F. Tzeng, H. C. Chen, J. Y. Guh, J. H. Tsai] Aims/hypothesis. Several investigations have shown that the renal medulla has a greater capacity to generate nitric oxide than the renal cortex. To further evaluate the changes of nitric oxide synthesis in the kidney, particularly in the outer medulla, in disorders involving fluid and electrolyte imbalances, we sought to determine renal nitric oxide synthase expression in the diabetic rats. Methods. We determined renal nitric oxide synthase mRNA and urinary nitrite/nitrate excretion in 12 normal and 12 streptozotocin-induced diabetic rats by reverse transcription-polymerase chain reaction with Southern blot hybridization and with Griess reaction, respectively. Nitric oxide synthase immunoreactivity was detected by immunohistochemistry in four normal and four diabetic rats. Results. Neuronal and endothelial nitric oxide synthase mRNA were 3.5-fold and 1.8-fold increased in the outer medulla of 12 diabetic rats with no difference found in the cortex and inner medulla when compared with 12 normal rats. Urinary nitrite/nitrate excretion was significantly increased from the first week after diabetic induction. In normal rats, immunohistochemical studies showed positive neuronal and endothelial nitric oxide synthase immunostaining in almost all segments of renal tubules. Diabetic rats had the greatest enhancement of immunostaining for neuronal and endothelial nitric oxide synthase in the proximal straight tubule and medullary thick ascending limb. Conclusion/interpretation. Our results indicate that increases in neuronal and endothelial nitric oxide synthase synthesis in the kidney, particularly in the outer medulla, possibly play an important part in the adaptation of renal function to hyperglycaemia and hyperosmolality in diabetes. [Diabetologia (2000) 43: 649-659] Springer-Verlag Berlin Heidelberg, 2000 Keywords Nitric oxide nationallicence neuronal nitric oxide synthase nationallicence endothelial nitric oxide synthase nationallicence diabetic kidney nationallicence outer medulla nationallicence renin nationallicence angiotensinogen nationallicence Shin S. J. Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut Lai F. J. Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut Wen J. D. Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut Hsiao P. J. Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut Hsieh M. C. Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut Tzeng T. F. Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut Chen H. C. Division of Nephrology, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut Guh J. Y. Division of Nephrology, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut Tsai J. H. Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut https://doi.org/10.1007/s001250051354 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051354 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shin S. J. Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut NATIONALLICENCE 700 1- Lai F. J. Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut NATIONALLICENCE 700 1- Wen J. D. Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut NATIONALLICENCE 700 1- Hsiao P. J. Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut NATIONALLICENCE 700 1- Hsieh M. C. Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut NATIONALLICENCE 700 1- Tzeng T. F. Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut NATIONALLICENCE 700 1- Chen H. C. Division of Nephrology, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut NATIONALLICENCE 700 1- Guh J. Y. Division of Nephrology, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut NATIONALLICENCE 700 1- Tsai J. H. Division of Endocrinology and Metabolism, Kaohsiung Medical University, Kaohsiung, Taiwan, TW aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer