caa a22 4500 475772881 CHVBK 20180406123622.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001250050035 doi (NATIONALLICENCE)springer-10.1007/s001250050035 Albumin permeability in isolated glomeruli in incipient experimental diabetes mellitus [Elektronische Daten] [M. Carraro, W. Mancini, M. Artero, C. Zennaro, L. Faccini, R. Candido, L. Armini, M. Calci, R. Carretta, B. Fabris] Aims/hypothesis. The pre-clinical phase of diabetic nephropathy is characterised by increased glomerular filtration rate and episodes of microalbuminuria. The cause of the microalbuminuria has been variably ascribed to alterations of the size or charge selective barriers of the glomerulus or both or as a consequence of the haemodynamic changes. Our aim was to investigate very early albumin permeability alterations in isolated glomeruli which were not subject to perfusion pressure.¶Methods. Isolated glomeruli were studied from 120 male Wistar rats, divided into three groups: streptozotocin-treated, streptozotocin-treated with insulin pellet implants, and controls. From each group ten animals were killed at 7, 14, 28, and 56 days after induction. Study variables included blood pressure, proteinuria, iopamidol clearance, albumin permeability and glomerular area. Subsequently, albumin permeability, proteinuria, and iopamidol clearance were determined in an additional group of 40 diabetic animals studied at 24, 72, 96, and 120 h after induction.¶Results. Albumin permeability increased steadily from induction in streptozotocin-treated animals, reaching a plateau at approximately 120 h. Glomerular filtration rate was shown to increase significantly at approximately 7 days and proteinuria correlated with it. Glomerular hypertrophy was observed both in streptozotocin-treated animals and in streptozotocin-treated rats with insulin pellet implants. Strict blood glucose control delayed the appearance of the permeability defect in isolated glomeruli and inhibited the increase in glomerular filtration in intact animals. It did not prevent glomerular hypertrophy.¶Conclusion/interpretation. An albumin permeability defect exists early in isolated non-perfused glomeruli from streptozotocin-treated rats and seems to be independent of glomerular filtration rate alterations. [Diabetologia (2000) 43: 235-241] Springer-Verlag Berlin Heidelberg, 2000 Keywords Diabetic nephropathy, albumin permeability, streptozotocin, blood glucose, proteinuria nationallicence Carraro M. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut Mancini W. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut Artero M. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut Zennaro C. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut Faccini L. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut Candido R. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut Armini L. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut Calci M. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut Carretta R. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut Fabris B. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut https://doi.org/10.1007/s001250050035 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250050035 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carraro M. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut NATIONALLICENCE 700 1- Mancini W. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut NATIONALLICENCE 700 1- Artero M. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut NATIONALLICENCE 700 1- Zennaro C. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut NATIONALLICENCE 700 1- Faccini L. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut NATIONALLICENCE 700 1- Candido R. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut NATIONALLICENCE 700 1- Armini L. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut NATIONALLICENCE 700 1- Calci M. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut NATIONALLICENCE 700 1- Carretta R. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut NATIONALLICENCE 700 1- Fabris B. Institute of Clinical Medicine, University of Trieste, Trieste, Italy, IT aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer