caa a22 4500 475773039 CHVBK 20180406123623.0 cr unu---uuuuu 170329e20000201xx s 000 0 eng 10.1007/s001250050027 doi (NATIONALLICENCE)springer-10.1007/s001250050027 The tumour necrosis factor alpha -238 G → A and -308 G → A promoter polymorphisms are not associated with insulin sensitivity and insulin secretion in young healthy relatives of Type II Diabetic patients [Elektronische Daten] [M. Koch, K. Rett, A. Volk, E. Maerker, K. Haist, M. Weisser, A. Rettig, W. Renn, H. U. Häring] Aims/hypothesis. Tumour necrosis factor-α (TNF-α) is believed to influence skeletal muscle insulin resistance. Two G → A transitions in the promoter region of TNF- α at position -238 and -308 have been identified that could play a part in transcriptional regulation of the gene. Insulin resistance is an independent familial trait that predicts the development of Type II (non-insulin-dependent) diabetes mellitus. We investigated the influence on insulin sensitivity and insulin secretion of both polymorphisms in a cohort of young healthy relatives of patients with Type II diabetes.¶Methods. We examined 109 first-degree relatives of Caucasian patients with a history of Type II diabetes, who underwent extensive metabolical and anthropometrical phenotyping, and determined the TNF- α -238 and -308 G→ A promoter polymorphisms.¶Results. For the -238 polymorphism, 83 probands (76.1 %) were homozygous for the G-allele, 25 probands (22.9 %) were heterozygous and 1 proband (0.9 %) was homozygous for the A-allele. For the -308 polymorphism, 83 probands (76.1 %) were homozygous for the G-allele, 24 probands (22.0 %) were heterozygous and 2 probands (1.18 %) were homozygous for the A-allele. Probands with and without the polymorphism did not differ in insulin sensitivity (p = 0.78), insulin-concentrations and C-peptide concentrations in oral glucose tolerance tests (p > 0.05).¶Conclusions/interpretation. We could not detect an association between insulin sensitivity or insulin secretion and TNF- α promoter polymorphisms in our cohort. The polymorphisms occur at the same frequencies in probands with either low or high insulin sensitivity. [Diabetologia (2000) 43: 181-184] Springer-Verlag Berlin Heidelberg, 2000 Keywords Insulin resistance, Type II diabetes, tumour necrosis factor-α, promotor polymorphisms, first-degree relatives nationallicence Koch M. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut Rett K. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut Volk A. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut Maerker E. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut Haist K. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut Weisser M. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut Rettig A. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut Renn W. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut Häring H. U. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut https://doi.org/10.1007/s001250050027 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250050027 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Koch M. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut NATIONALLICENCE 700 1- Rett K. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut NATIONALLICENCE 700 1- Volk A. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut NATIONALLICENCE 700 1- Maerker E. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut NATIONALLICENCE 700 1- Haist K. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut NATIONALLICENCE 700 1- Weisser M. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut NATIONALLICENCE 700 1- Rettig A. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut NATIONALLICENCE 700 1- Renn W. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut NATIONALLICENCE 700 1- Häring H. U. Medical Clinic of the University of Tübingen, Section IV, Internal Medicine and Endocrinology, Tübingen, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer