caa a22 4500 475773144 CHVBK 20180406123623.0 cr unu---uuuuu 170329e20001001xx s 000 0 eng 10.1007/s001250051551 doi (NATIONALLICENCE)springer-10.1007/s001250051551 Tetrahydrobiopterin improves endothelium-dependent vasodilation by increasing nitric oxide activity in patients with Type II diabetes mellitus [Elektronische Daten] [T. Heitzer, K. Krohn, S. Albers, T. Meinertz] Aims/hypothesis. Tetrahydrobiopterin is an essential cofactor of nitric oxide synthase, and its deficiency decreases nitric oxide bioactivity. Our aim was to find whether supplementation of tetrahydrobiopterin could improve endothelial dysfunction in diabetic patients.¶Methods. Forearm blood flow responses to the endothelium-dependent vasodilator acetylcholine (0.75- 3.0 μg · 100 ml-1· min-1) and to the endothelium-independent vasodilator sodium nitroprusside (0.1-1.0 μg · 100 ml-1· min-1) before and during concomitant intra-arterial infusion of tetrahydrobiopterin (500 μg/min) were measured by venous occlusion plethysmography in 12 control subjects and 23 patients with Type II (non-insulin-dependent) diabetes mellitus.¶Results. In control subjects, tetrahydrobiopterin had no effect on the dose-response curves to acetylcholine and sodium nitroprusside. In contrast, in diabetic patients, the attenuated endothelium-dependent vasodilation to acetylcholine was considerably improved by concomitant treatment with tetrahydrobiopterin, whereas the endothelium-independent vasodilation was not affected. This beneficial effect of tetrahydrobiopterin in diabetic patients could be completely blocked by N G-monomethyl-l-arginine.¶Conclusion/interpretation. These findings suggest the possibility that endothelial dysfunction in Type II diabetes might be related to decreased availability of tetrahydrobiopterin. [Diabetologia (2000) 43: 1435-1438] Springer-Verlag Berlin Heidelberg, 2000 Keywords Diabetes mellitus, endothelium, nitric oxide, tetrahydrobiopterin, endothelium-dependent vasodilation, acetylcholine, NG-monomethyl-l-arginine nationallicence Heitzer T. University Hospital Clinic Hamburg-Eppendorf, Department of Cardiology, Clinic for Internal Medicine, Hamburg, Germany, DE aut Krohn K. University Hospital Clinic Hamburg-Eppendorf, Department of Cardiology, Clinic for Internal Medicine, Hamburg, Germany, DE aut Albers S. University Hospital Clinic Hamburg-Eppendorf, Department of Cardiology, Clinic for Internal Medicine, Hamburg, Germany, DE aut Meinertz T. University Hospital Clinic Hamburg-Eppendorf, Department of Cardiology, Clinic for Internal Medicine, Hamburg, Germany, DE aut https://doi.org/10.1007/s001250051551 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s001250051551 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heitzer T. University Hospital Clinic Hamburg-Eppendorf, Department of Cardiology, Clinic for Internal Medicine, Hamburg, Germany, DE aut NATIONALLICENCE 700 1- Krohn K. University Hospital Clinic Hamburg-Eppendorf, Department of Cardiology, Clinic for Internal Medicine, Hamburg, Germany, DE aut NATIONALLICENCE 700 1- Albers S. University Hospital Clinic Hamburg-Eppendorf, Department of Cardiology, Clinic for Internal Medicine, Hamburg, Germany, DE aut NATIONALLICENCE 700 1- Meinertz T. University Hospital Clinic Hamburg-Eppendorf, Department of Cardiology, Clinic for Internal Medicine, Hamburg, Germany, DE aut Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer